We narrowed to 13,713 results for: TIM
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Plasmid#191450PurposeVisualizing endogenous & active Cdc42DepositorInsertWASp
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFC330
Plasmid#87842PurposeAMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection markerDepositorInsertsCas9
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup88
Plasmid#87338PurposeTo express human Nup88 in mammalian cellsDepositorInsertNUP88 (NUP88 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
#1.4 post-G-SynapShot-mScarlet
Plasmid#214716PurposeExpresses green #1.4 post-SynapShot and mScarletDepositorInsert#1.4 post-G-SynapShot
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-21(+)Tm9D8* TrpB
Plasmid#207517PurposeExpression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serineDepositorInsertTryptophan synthase β-subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt-mTagBFP2-P2A-mScarlet
Plasmid#196115Purposezebrafish expression of nbt-mTagBFP2-P2A-mScarletDepositorInsertnbt-mTagBFP2-P2A-mScarlet
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJM.657
Plasmid#108525PurposeCymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.DepositorInsertCymRAM + PCymRC-YFP
UseTagsExpressionBacterialMutationCymR: S110G A171VPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJ915
Plasmid#69090PurposeExpress Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLSDepositorInsertcas9
UseTagsMBP, hexa His, and two SV40 NLSExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414-TEF1p-Cas9-CYC1t
Plasmid#43802PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
Plasmid#63166PurposeGAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporterDepositorInsert"spaghetti monster GFP-HA"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYL-kasOp-FnCas12a2
Plasmid#177874PurposeExpresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.DepositorInsertStreptomyces codon optimized FnCas12a
UseCRISPRTagsExpressionMutationPromoterkasOp*Available sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP35_MARV
Plasmid#135471PurposeEncodes Marburg virus VP35 gene under control of the AG promoterDepositorInsertVP35
UseTagsExpressionMammalianMutationNonePromoterAGAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_NP_MARV
Plasmid#135470PurposeEncoding Marburg virus NP gene under control of the AG promoterDepositorInsertnucleoprotein (NP)
UseTagsExpressionMammalianMutationNonePromoterAGAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pActin-Electra2
Plasmid#184942Purposebeta-actin labelling with blue fluorescent protein Electra2DepositorInsertElectra2-beta-actin
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAJM.677
Plasmid#108530PurposeAraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.DepositorInsertAraCAM + AraE + PBAD-YFP
UseTagsExpressionBacterialMutationAraC: L133F E165L E169G C280LPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYL-kasOp-FnCas12a3
Plasmid#177875PurposeExpresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.DepositorInsertStreptomyces codon optimized the FnCas12a mutant EP16
UseCRISPRTagsExpressionMutationPromoterkasOp*Available sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBV-Luc
Plasmid#71414PurposeTo see the activity of HBV transcription using Luciferase assayDepositorInsertHBV core promoter, enhancer I, enhancer II and luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterHBVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR4 hNFATc1-EE
Plasmid#17857DepositorInsertNFATc1 (NFATC1 Human)
UseTagsEEExpressionBacterialMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
mCherry- eMagBF - 5ptaseOCRL
Plasmid#162254PurposeBlue-light dependent heterodimerization systemDepositorInsertmCherry- eMagBF - 5ptaseOCRL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-MTS
Plasmid#124315PurposeExpresses mitochondria specific HaloTag proteinDepositorInsertHalo tag
UseTagsMito specific seqExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green
Plasmid#170048PurposeTri-Responsive Reporter Gene Construct for redgreen, triple red, and double greenDepositorInsertsmScarlet
COmScarlet
mRuby3
mWasabi dN
mWasabi CO
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G2
Plasmid#40175DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCA14-pHR-SFFV-GFP-RPA43
Plasmid#199445PurposeExpression of GFP-RPA43 in mammalian cellsDepositorInsertRPA43 (POLR1F Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterSFFVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJM.474
Plasmid#108526PurposeLuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.DepositorInsertLuxR + PLuxB-YFP
UseTagsExpressionBacterialMutationVanR: T49I A111V P179SPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-mCD8
Plasmid#162747PurposeNFAT-driven ZsGreen-1 reporter gene with mouse CD8 geneDepositorInsert8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luke-2
Plasmid#228900PurposeLuke-2 localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertCarbohydrate binding domain cbm49 protein
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
#2.4 pre-G-SynapShot-Myc
Plasmid#214722PurposeExpresses green #2.4 pre-SynapShot and Myc tagDepositorInsert#2.4 pre-G-SynapShot
UseAAVTagsMycExpressionMammalianMutationPromoterCAGAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mWatermelon
Plasmid#190625PurposeBacterial expression of mWatermelon GFPDepositorInsertmWatermelon
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse cyclin D2
Plasmid#86791Purposebacterial expression of mouse cyclin D2DepositorInsertcyclin D2 (Ccnd2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAR-mOrange2-sfGFP
Plasmid#115693PurposeGeneration of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent TimerDepositorInsertSA-mOrange2-sfGFP-SD
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV_ADRB2-mTurquoise2-iLID
Plasmid#161002PurposeMammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusionDepositorInsertADRB2-mTurquoise2-iLID
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-sfGFPx2
Plasmid#51559PurposeSuperfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)DepositorInsertsuperfolder GFP
UseTagsGLESTCRHASLAVLADERRFSA and MARARAExpressionBacterialMutationContains additonal aa in the N and C terminus (in…PromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPaxillin-Electra1-N1
Plasmid#179484PurposeExpresses paxillin fused to blue fluorescent protein Electra1DepositorInsertpaxillin-Electra1
UseTagsElectra1ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LEPZ
Plasmid#111161PurposemiR-E (miR-30 variant)-based RNAi with ZsGreen reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPIDNB2
Plasmid#169902PurposeEmpty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mNeonGreen is fused to H2B to allow for visualization of the nucleus.DepositorTypeEmpty backboneUseAvian expressionTagsExpressionMammalianMutationPromoterDox inducibleAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
LT3GECIR
Plasmid#111178PurposeTet-ON miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterT3GAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
PE-P3-rice
Plasmid#185612PurposeFor plant prime editing in rice plantsDepositorInsert3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
ShuttleNSE
Plasmid#50958PurposeA backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoterDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterNSEAvailable sinceFeb. 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Akaluc-N1
Plasmid#120371PurposeAkaluc luciferase mammalian expression in -N1 backboneDepositorInsertAkaluc (human optimized)
UseTagsExpressionMammalianMutationHuman codon optimizedPromoterCMVAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-mCherry-XL (R125K)
Plasmid#188054PurposeExpresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteriaDepositorInsertmCherry-XL (R125K)
UseTags6xHisExpressionBacterialMutationdiffers from mCherry by R125K, W143S, I161V, Q163…PromoteraraBADAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CoChR-mRuby2-ST
Plasmid#109131PurposeCation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertCoChR-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.771
Plasmid#108534PurposeNahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.DepositorInsertNahRAM + PSalTTC-YFP
UseTagsExpressionBacterialMutationNahR: I25V P72H V118A R248CPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZR060_CROPseq-sgCtrl-CRISPRi
Plasmid#180269PurposeLentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRiDepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_gRNA
Plasmid#80457PurposegRNA expression vector with mCherry transfection controlDepositorInsertssp Cas9 gRNA
mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterU6 and chicken β-actin promoterAvailable sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mKate2
Plasmid#104009PurposePlasmid encoding mKate2DepositorInsertmKate2
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, V45A, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-PAmCherry1-MCS
Plasmid#60608PurposeCloning PAmCherry1DepositorInsertPAmCherry1
UseCloningTagsExpressionMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lys-eMagB-iRFP
Plasmid#162249PurposeBlue-light dependent heterodimerization systemDepositorInsertp18-eMagB-iRFP
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-CmCHS-MsCHI
Plasmid#73394PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
CmCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only