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pDEST40-CES2C-ΔC

Plasmid

#200555

Purpose

Mammalian expression of secreted mouse CES2C

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)

Use

Tags

6xHis, Flag, and V5

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2

Plasmid

#196534

Purpose

Expression of GST-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Opa1

Plasmid

#191961

Purpose

Gateway Destination Vector, containing OPA1 coding sequence, for Gateway Cloning Technology

Depositor

Luca Scorrano

Insert

Opa1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV2-DEST(r)

Plasmid

#190238

Purpose

Gateway destination vector, empty AAV vector backbone

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_F3194S

Plasmid

#187140

Purpose

mammalian cell expression of FLAG-HUWE1 F3194S

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

F3194S

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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GGDest1-Amp

Plasmid

#157649

Purpose

Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

none

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GGDest2-Amp

Plasmid

#157650

Purpose

Golden Gate assembly of a pET28-compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

none

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GGDest3-Amp

Plasmid

#157651

Purpose

Golden Gate assembly of a pTXB1 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

none

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GGDestX2-Amp

Plasmid

#157654

Purpose

Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6, CBh

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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CSIV-DEST

Plasmid

#141053

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pFS_0376_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1_RC

Plasmid

#116988

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0380_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3_RC

Plasmid

#116992

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0366_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_2_RC

Plasmid

#116978

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0363_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_1

Plasmid

#116975

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0379_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3

Plasmid

#116991

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0346_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1_RC

Plasmid

#116958

Purpose

Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Bacteroides fragilis strain S14 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0365_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_2

Plasmid

#116977

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0364_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_1_RC

Plasmid

#116976

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0345_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1

Plasmid

#116957

Purpose

Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Bacteroides fragilis strain S14 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0377_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2

Plasmid

#116989

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge