We narrowed to 17,080 results for: EMB

1. pEGFP-N1 Dynamin1 D406R M407R

   Plasmid

   #112109

   Purpose

   Mammalian expression plasmid of GFP-tagged Dynamin protein.

   Depositor

   Justin Taraska

   Article

   Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

   Insert

   Dynamin1 (DNM1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D406R M407R

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1 Dynamin1 D406R M407R F409A

   Plasmid

   #112110

   Purpose

   Mammalian expression plasmid of GFP-tagged Dynamin protein.

   Depositor

   Justin Taraska

   Article

   Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

   Insert

   Dynamin1 (DNM1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D406R M407R F409A

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

   Plasmid

   #65846

   Purpose

   Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R5A-K6A

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP

   Plasmid

   #65847

   Purpose

   Mammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R5D-K6D

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1

   Plasmid

   #42185

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

   Insert

   G protein Gamma 3(F70A, F71A) (Gng3 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   F70A, F71A

   Promoter

   cmv

   Available Since

   March 26, 2013

   Availability

   Academic Institutions and Nonprofits only

6. EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1

   Plasmid

   #42188

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

   Insert

   Gamma 3(K68A,K69A) (Gng3 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K68A,K69A

   Promoter

   cmv

   Available Since

   March 26, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pSN-A112C-GpA-G83I

   Plasmid

   #191239

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
   

   Tags

   nuclease A from S. aureus fused to the TM domain …

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and Glycin…

   Promoter

   T7 promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1 _Gata6

   Plasmid

   #73537

   Purpose

   Inducible lentiviral expression of Gata6

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   Gata6 (Gata6 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 2C-EGFP-t2a-mCD4

   Plasmid

   #216213

   Purpose

   Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

   Depositor

   Michelle Percharde

   Article

   Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

   Insert

   CD4 (Cd4 Mouse)
   

   Tags

   GFP-T2A

   Expression

   Mammalian

   Mutation

   CD4 transmembrane domain only

   Promoter

   MERVL

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1A-LivePAR(Y107A)-Hygro

    Plasmid

    #176073

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    LivePAR (Y107A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation to convert Try107 to Ala

    Promoter

    EF1A

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE80L-SpyCatcher-ELP-GFP

    Plasmid

    #69835

    Purpose

    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

    Insert

    SpyCatcher-ELP-GFP
    

    Tags

    6x His Tag, GFP, and TEV Tag

    Expression

    Bacterial

    Promoter

    T5 promoter/lac operator element

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1-Cdx2-3xT7

    Plasmid

    #200891

    Purpose

    Inducible lentiviral expression of Cdx2

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Cdx2 (Cdx2 Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENT-L1-Kozak-GAP43-ECFP-stop-L2

    Plasmid

    #186356

    Purpose

    Entry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Growth-associated protein-43 (Gap43 Mouse, Synthetic)
    

    Use

    Expression of a fluorescent membrane marker

    Tags

    ECFP

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CaMKIIa-SpyTag-C1C2-mCherry

    Plasmid

    #69833

    Purpose

    Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

    Insert

    SpyTag-C1C2
    

    Use

    Lentiviral

    Tags

    Trafficking signal and mCherry

    Expression

    Mammalian

    Mutation

    Inserted SpyTag after the signal peptide of C1C2.

    Promoter

    CamKIIa

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKI-cjFOXP2-hum

    Plasmid

    #129757

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    FOXP2 (LOC100411307 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Mutation

    T301N/N323S

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-neo-myc-LC3(deltaC22, G120A)

    Plasmid

    #45449

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Encodes amino acids 1-120, Glycine at position 12…

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. CD8a-hnRNP-M-EGFP

    Plasmid

    #86054

    Purpose

    A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    CD8a (CD8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CD8a luminal and transmembrane domians along with…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CD8a-cNLS-EGFP

    Plasmid

    #86052

    Purpose

    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    CD8a (CD8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CD8a luminal and transmembrane along with two SV4…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFP G protein Gamma 3(K68A,K69A,F70A,F71A) in pcDNA3.1

    Plasmid

    #42189

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    Gamma 3(K68A,K69A,F70A,F71A) (Gng3 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K68A,K69A,F70A,F71A

    Promoter

    CMV

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

20. EGFP G protein Gamma 3(A73L,L74I,L75S) in pcDNA3.1

    Plasmid

    #42190

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    G protein Gamma 3(A73L,L74I,L75S) (Gng3 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A73L,L74I,L75S

    Promoter

    CMV

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only