Addgene

We narrowed to 5,095 results for: PEP

Showing: 1021 - 1040 of 5095 results

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SAPDF-pET21a(+)

Plasmid

#12649

Depositor

SeWon Suh

Article

Yoon et al Proteins. 2004 Nov 15. 57(3):639-42.

Insert

SA Peptide deformylase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Aq(2nd)

Plasmid

#190911

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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V96 ELP

Plasmid

#68392

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V96 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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V72 ELP

Plasmid

#68938

Purpose

Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

Depositor

Andrew MacKay

Article

Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

Insert

V72 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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NM R2E2 GFP

Plasmid

#1208

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 NM domain (SUP35 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

2 additional copies of second oligopeptide repeat…

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pET28-Butelase

Plasmid

#221848

Purpose

Expresses asparaginyl endopeptidase butelase

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

Butelase 1 ligase

Use

Tags

Expression

Bacterial

Mutation

No

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-Empty-cleavableELP

Plasmid

#219401

Purpose

Insert protease recognition site with BsaI to make custom protease-responsive elastin-like polypeptide (ELP)

Depositor

Danielle Mai

Article

Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

Insert

Empty-cleavableELP

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-mCherry(-6)

Plasmid

#205045

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-mCherry(-6)-L1

Plasmid

#205046

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)-L1

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-mCherry(-6)-L2

Plasmid

#205047

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)-L2

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-mCherry(-6)-L3

Plasmid

#205048

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)-L3

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-L1

Plasmid

#205027

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-L1

Use

Tags

KGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-L2

Plasmid

#205028

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-L2

Use

Tags

KGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-L3

Plasmid

#205029

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-L3

Use

Tags

KGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-H1

Plasmid

#205030

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H1

Use

Tags

KKRRRKK and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-H2

Plasmid

#205031

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2

Use

Tags

KKRRRKKKRRRKK and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-H3

Plasmid

#205032

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H3

Use

Tags

KKRRRKKKRRRKKKRRRKK and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pYAL_HLA-A2_MART1

Plasmid

#171177

Purpose

Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

Depositor

Michael Birnbaum

Article

Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

Insert

MART1_HLA-A2*01 single-chain trimer fusion

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBA2219

Plasmid

#38152

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

DsbC (dsbC E. coli)

Use

Tags

N-terminal leader peptide (cloning artifact)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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MARS

Plasmid

#205232

Purpose

Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells

Depositor

Jeremy Baskin

Insert

PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)

Use

Tags

Nuclear Export Sequence and mScarlet-i

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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