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pMElmo2P1gfp

Plasmid

#90127

Purpose

Gateway Mid-entry clone containing promoter sequence specific for lmo2 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2P1Egfp (lmo2 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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p5Elmo2E1

Plasmid

#90118

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of lmo2

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2E1 (lmo2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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p5Edusp5E1

Plasmid

#90122

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of dusp5

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5E1 (dusp5 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dusp5P1Egfp

Plasmid

#90161

Purpose

contains dusp5 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5P1Egfp (dusp5 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMEdusp5P1gfp

Plasmid

#90126

Purpose

Gateway Mid-entry clone containing promoter sequence specific for dusp5 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5P1Egfp (dusp5 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 Q147A, M151A, I154A

Plasmid

#74616

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Q147A, M151A, I154A

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 D102A, E108A

Plasmid

#74618

Purpose

Expresses a double mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 N68A, W69A, K77A

Plasmid

#74619

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pT7II-2N4Rtau

Plasmid

#177653

Purpose

Recombinant protein synthesis of human Tau

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

Tau (2N4R) (MAPT Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pFBDM

Plasmid

#110738

Purpose

Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin and p10 promoters

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-pHuji

Plasmid

#61556

Purpose

Red fluorescent protein based pH indicator in pDisplay vector

Depositor

Robert Campbell

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHuji

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pSPL

Plasmid

#110743

Purpose

Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin and p10 promoters

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pUCDM

Plasmid

#110742

Purpose

Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin and p10 promoters

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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fabp10a:GCaMP6s; cryaa:mCherry

Plasmid

#230060

Purpose

Expression of genetically encoded calcium indicator in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Pozo-Morales et al Hepatology. 2023 Mar 1;77(3):789-801. do

Insert

GCaMP6s

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

fabp10a

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-pHuji

Plasmid

#61555

Purpose

Red fluorescent protein based pH indicator in bacterial expression vector

Depositor

Robert Campbell

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHuji

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pKESK22

Plasmid

#64857

Purpose

expression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replication

Depositor

Karin Schnetz

Article

Stratmann et al J Bacteriol. 2008 Feb;190(3):926-35. Epu

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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GG-dest

Plasmid

#69538

Purpose

Destination plasmid for gRNA concatenation Golden Gate cloning

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-pHoran4

Plasmid

#61557

Purpose

Orange fluorescent protein based pH indicator in pDisplay vector

Depositor

Robert Campbell

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHoran4

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pBAD-pHoran4

Plasmid

#61558

Purpose

Orange fluorescent protein based pH indicator in bacterial expression vector

Depositor

Robert Campbell

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHoran4

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pET-6xHis-mCherry(-6)

Plasmid

#205045

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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