We narrowed to 1,270 results for: grna cloning vector

1. pLV-PGK-DIO-mCherry-nega miR

   Plasmid

   #137732

   Purpose

   Lentivirus vector for control miR in a Cre-dependent manner

   Depositor

   Tomoyuki Furuyashiki

   Article

   Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

   Insert

   control microRNA
   

   Use

   Lentiviral

   Tags

   mChherry

   Expression

   Mammalian

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

   Plasmid

   #171379

   Purpose

   Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized Cas13X.1
   

   Expression

   Mammalian

   Promoter

   CMV, U6

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSIREN-RetroQ-HSPE-sh1

   Plasmid

   #92033

   Purpose

   pSIREN-RetroQ vector containing shRNA sequence to HPSE

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA #1 to HPSE (Hpse Mouse)
   

   Use

   Retroviral

   Available Since

   July 19, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSIREN-RetroQ-HSPE-sh2

   Plasmid

   #92034

   Purpose

   pSIREN-RetroQ vector containing shRNA sequence to HPSE

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA #2 to HPSE (Hpse Mouse)
   

   Use

   Retroviral

   Available Since

   July 19, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. c3GIC9n

   Plasmid

   #62192

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9 (D10A)
   

   Use

   CRISPR

   Tags

   HA

   Mutation

   D10A substitution (Cas9n)

   Promoter

   TRE3G

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-IRF4-Puro

   Plasmid

   #123323

   Purpose

   Lentiviral vector for expression of IRF4 under a Doxycycline inducible promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

   Insert

   IRF4 (IRF4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   several silent mutations to disrupt sgRNA targeti…

   Promoter

   Doxycycline inducible minimal CMV

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-(BsaI)_CBh-Cas9

   Plasmid

   #135010

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Tags

   3xFLAG (N terminal on insert)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEIF3D-RNAgateDel-sgEIF3D

   Plasmid

   #222137

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   V291-F306 deletion

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEIF3D-NtermDel-sgEIF3D

   Plasmid

   #222123

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   S161 truncation

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEIF3D-a11helixDel-sgEIF3D

    Plasmid

    #222135

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    T317E, N320E, H321A

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEIF3D-CtermDel-sgEIF3D

    Plasmid

    #222125

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    F527 truncation

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEIF3D-a5helixDel-sgEIF3D

    Plasmid

    #222133

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    D249Q, V262I, Y263A

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCROPseq-VEX-optimized-scaffold

    Plasmid

    #203321

    Purpose

    Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEIF3D-S528D-S529D-sgEIF3D

    Plasmid

    #222127

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    S528D, S529D

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEIF3D-NtermDel-CtermDel-sgEIF3D

    Plasmid

    #222131

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    161 + 527 truncation

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEIF3D-S528N-S529N-sgEIF3D

    Plasmid

    #222129

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    S528N, S529N

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXS-IRES-Blast ACSL4

    Plasmid

    #173164

    Purpose

    Retroviral vector to express sgRNA resistant human ACSL4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
    

    Use

    Retroviral

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-Blast HA-GPAT4

    Plasmid

    #173168

    Purpose

    Retroviral vector to express sgRNA resistant HA tagged human GPAT4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXS-IRES-Blast mAtg7

    Plasmid

    #173170

    Purpose

    Retroviral vector to express sgRNA resistant mouse Atg7

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available Since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXS-IRES-Blast 3XFLAG-CHP1

    Plasmid

    #173166

    Purpose

    Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
    

    Use

    Retroviral

    Tags

    3XFLAG

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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