We narrowed to 6,573 results for: tac

1. pFM108

   Plasmid

   #40154

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant, S241A (par-2 Nematode)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S241A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   tac

   Available since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pFM106

   Plasmid

   #40152

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant, R163A (par-2 Nematode)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   R163A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   tac

   Available since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pFM105

   Plasmid

   #40151

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant, K162A (par-2 Nematode)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K162A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   tac

   Available since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

4. Pmyo-3::hpo-30 gDNA (pBAB204)

   Plasmid

   #117385

   Purpose

   Allows for body-wall muscle expression of HPO-30

   Depositor

   Kavita Babu

   Article

   Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

   Insert

   genomic DNA of hpo-30 (833 bp)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRPN265NB-enh-MNase

   Plasmid

   #166035

   Purpose

   Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

   Depositor

   Marc Timmers

   Article

   Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

   Insert

   Glutathione-S-Transferase/enhancer/MNase
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   lac promoter / tac promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-2T_EV-D68_VP1

   Plasmid

   #202072

   Purpose

   Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   EV-D68 VP1 protein with Histag
   

   Use

   Tags

   6xHis-tag and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pES002 MBP-GSK3β_S9A-HA-His

   Plasmid

   #196184

   Purpose

   Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Insert

   GSK3B (GSK3B Human)
   

   Use

   Tags

   HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   changed Serine 9 to Alanine

   Promoter

   Tac

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMAL-MBP-Cdc42-Q61L

   Plasmid

   #214288

   Purpose

   Express constitutively active Cdc42 fused with MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Constitutively active Cdc42 (CDC42 Human)
   

   Use

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   Cdc42-Q61L

   Promoter

   Tac

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX4T-hLIG3

   Plasmid

   #81055

   Purpose

   bacterial expression of human LIG3

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   DNA ligase 3 (LIG3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX4T3-GST-EGFP

    Plasmid

    #177136

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX nsp1 CoV2

    Plasmid

    #175512

    Purpose

    For protein expression of WT nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-parkin 141-465

    Plasmid

    #45971

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 141-465 (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTSSX-STC

    Plasmid

    #115668

    Purpose

    E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

    Depositor

    David Kramer

    Article

    Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

    Insert

    small tetraheme cytochrome c
    

    Use

    Tags

    Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GST-MAGE-2-429

    Plasmid

    #89129

    Purpose

    bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

    Depositor

    Elizabeth Wilson

    Article

    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pPA016 (delta 86-130 Parkin)

    Plasmid

    #102357

    Purpose

    expresses Rat Parkin with residues 86-130 deleted

    Depositor

    Kalle Gehring

    Article

    Sauve et al EMBO J. 2015 Oct 14;34(20):2492-505. doi

    Insert

    Parkin (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted residues 86 to 130

    Promoter

    tac

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SRSF9_2xRRM_pGEX

    Plasmid

    #135120

    Purpose

    Recombinant expression of SRSF9 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    SRSF9 (SRSF9 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX SHIP2-SH2

    Plasmid

    #46493

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHIP2
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 8-131

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. SRSF8_RRM_pGEX

    Plasmid

    #135119

    Purpose

    Recombinant expression of SRSF8 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    SRSF8 (SRSF8 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-human MIZ1 wild type

    Plasmid

    #74161

    Purpose

    bacterial expression of MIZ1

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MIZ1 (ZBTB17 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pGEX-6p-hDcp2 E147/8Q

    Plasmid

    #72217

    Purpose

    bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E147Q, E148Q and F404L (please see depositor'…

    Promoter

    tac

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only