We narrowed to 77,445 results for: myc

1. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

   Plasmid

   #227086

   Purpose

   Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Use

   Tags

   GST-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   R1282H

   Promoter

   T7

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

   Plasmid

   #227084

   Purpose

   Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Use

   Tags

   STREPii-FLAG and SV40 NLS 1

   Expression

   Yeast

   Mutation

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CUP1-∆ssCPY*-mScarletI

   Plasmid

   #221157

   Purpose

   Fluorescent reporter for cytoplasmic aggregates

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Use

   Tags

   mScarletI

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CUP1-∆ssCPY*-mNeonGreen

   Plasmid

   #221156

   Purpose

   Fluorescent reporter for cytoplasmic aggregates

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Use

   Tags

   mNeonGreen

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)

   Plasmid

   #219690

   Purpose

   Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP

   Plasmid

   #211343

   Purpose

   Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection

   Depositor

   Monkol Lek

   Insert

   m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAMW-Wnk

   Plasmid

   #203978

   Purpose

   Myc6 Wnk under actin promoter for cell culture

   Depositor

   Andreas Jenny

   Insert

   Wnk (Wnk Fly)
   

   Use

   Tags

   6xMyc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

   Plasmid

   #182346

   Purpose

   PacI-flanked CAG-LSL expression cassette with a FNF for positive selection

   Depositor

   Mario Capecchi

   Insert

   tdTomato
   

   Use

   Mouse Targeting; Flp / frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB2HoptX

   Plasmid

   #163832

   Purpose

   Inactive two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B

   Depositor

   Jerome Fox

   Insert

   SpecR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   MidT Substrate Y4F

   Promoter

   Pro1;ProD;pLacZ-Opt

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNSU299_RPL39

    Plasmid

    #166078

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNSU299_YDR344C

    Plasmid

    #166072

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNSU299_TDH3

    Plasmid

    #166084

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Promoter of TDH3 (TDH3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid

    #160640

    Purpose

    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

    Depositor

    Diego Orzaez

    Insert

    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. 32 pCOLI_S_HA

    Plasmid

    #160174

    Purpose

    N-term HA 3C cleavable Spec resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    3xHA and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 29 pCOLI_S_C_STREP

    Plasmid

    #160171

    Purpose

    C-term 2xStrepII uncleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. 27 pCOLI_S_C_HIS

    Plasmid

    #160169

    Purpose

    C-term 8xHis uncleavable Spec resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV-gRNA-BTRC-MGMT_2

    Plasmid

    #136416

    Purpose

    Lentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.

    Depositor

    Massimo Squatrito

    Insert

    U6_sgRNA(BTRC)_U6_sgRNA(MGMT)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIAGO-aceS

    Plasmid

    #114456

    Purpose

    Expression of AceS methylase in streptomycetes

    Depositor

    Patrick Caffrey

    Article

    Sheehan et al Mol Biosyst. 2017 May 2;13(5):866-873. d

    Insert

    aceS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ermE

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAW8

    Plasmid

    #110222

    Purpose

    Cre-expression plasmid for cassette exchange

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. yCp50-PRP5

    Plasmid

    #111303

    Purpose

    Prp5 in yCp50 plasmid

    Depositor

    Christine Guthrie

    Article

    Noble et al Genetics. 1996 May;143(1):67-80.

    Insert

    PRP5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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