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TFORF0457

Plasmid

#142629

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TRIM24 (TRIM24 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Shim_AsnRS_419.110_SHI02794_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228373

Purpose

Expresses N-term transit peptide from Sapria himalayana Asparagine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 419.110 (SHI02794); determination of S. himalayana AsnRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana AsnRS evm.model.fragScaff scaffold 419.110 (SHI02794)

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_GlyRS_87.19_SHI04113_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228377

Purpose

Expresses N-term transit peptide from S. himalayana Glycine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 87.19 (SHI04113RA); determination of Sapria himalayana GlyRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana GlyRS evm.model.fragScaff scaffold 87.19 (SHI04113RA)

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_ProRS_3278.3.7_SHI28108_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228379

Purpose

Expresses N-term transit peptide from Sapria himalayana Proline aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA); determination of S. himalayana ProRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana ProRS evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA)

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Athal_PheRS_TP_pK7fWG2_IVD_FP611

Plasmid

#228370

Purpose

Expresses N-term transit peptide from Arabidopsis PheRS At3g58140 with demonstrated dual mito/plastid targeting; proof-of-concept for Plasmid 228371

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from Arabidopsis PheRS At3g58140 (Warren et al., 2023) with demonstrated dual mito/plastid targeting.

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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rag2:RAB3IP_CE_pISceI

Plasmid

#223044

Purpose

Express RAB3IP gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

RAB3IP (RAB3IP Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:WDR4_CE_pISceI

Plasmid

#223051

Purpose

Express WDR4 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

WDR4 (WDR4 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:TSFM_CE_pISceI

Plasmid

#223031

Purpose

Express TSFM gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

TSFM (TSFM Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:OS9_CE_pISceI

Plasmid

#223023

Purpose

Express OS9 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

OS9 (OS9 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:TSPAN31_CE_pISceI

Plasmid

#223025

Purpose

Express TSPAN31 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

TSPAN31 (TSPAN31 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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LCV2 PQBP1 KO2

Plasmid

#217448

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting PQBP1 (PQBP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA Q63/67A

Plasmid

#217439

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA Q63A,Q67A

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA Q67H/N74D

Plasmid

#217441

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA Q67H,N74D

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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LCV2 PQBP1 KO1

Plasmid

#217447

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting PQBP1 (PQBP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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PX4

Plasmid

#223340

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-EGFP-miniTurboID-3HA (PXA1 Mustard Weed)

Tags

EGFP-miniTurboID-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PX3

Plasmid

#223339

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-miniTurboID-EGFP-3HA (PXA1 Mustard Weed)

Tags

miniTurboID-EGFP-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PX2

Plasmid

#223338

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-EGFP-TurboID-3HA (PXA1 Mustard Weed)

Tags

EGFP-TurboID-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PX1

Plasmid

#223337

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-TurboID-EGFP-3HA (PXA1 Mustard Weed)

Tags

TurboID-EGFP-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-HA-CBS(opt)-FLAG

Plasmid

#230088

Purpose

expressing human CBS

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

CBS (CBS Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Multiple wobble mutations

Promoter

CMV

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF0089

Plasmid

#142532

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZEB1 (ZEB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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