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pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Plasmid

#195541

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.7 Na+ channel [N68/6R-1]

Plasmid

#206717

Purpose

Mammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.7 Na+ channel (Homo sapiens) recombinant Mouse monoclonal antibody (SCN9A Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-499/500R

Plasmid

#203575

Purpose

Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

silent point mutations to make resistant shRNA 49…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP

Plasmid

#210505

Purpose

expresses NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c

Plasmid

#210508

Purpose

expresses NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c

Tags

GB1

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-NTOM20-EGFP-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs

Plasmid

#210516

Purpose

expresses NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.7 Na+ channel [N68/6R-2b]

Plasmid

#206674

Purpose

Mammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.7 Na+ channel (Homo sapiens) recombinant Mouse monoclonal antibody (SCN9A Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgNotch2#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209069

Purpose

Entry vector that encodes sgRNAs against mouse Notch2, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

Insert

sgRNAs targeting Notch2, Rb1, Trp53, Rbl2 and Cre recombinase

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgNotch1#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209068

Purpose

Entry vector that encodes sgRNAs against mouse Notch1, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

Insert

sgRNAs targeting Notch1, Rb1, Trp53, Rbl2 and Cre recombinase

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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sh2g-ZMYND8-human

Plasmid

#201406

Purpose

Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

Insert

ZMYND8 (ZMYND8 Human)

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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sh4p-ZMYND8-mouse

Plasmid

#201405

Purpose

Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

Insert

ZMYND8

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

Plasmid

#184563

Purpose

CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

All lysines were mutated to arginines. Codon-opti…

Promoter

CMV

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgCdkn2c#2/Cre

Plasmid

#173584

Purpose

Expresses a Cdkn2c-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Cdkn2c (Cdkn2c Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgMga#2/Cre

Plasmid

#173602

Purpose

Expresses a Mga-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Mga (Mga Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgAtrx#2/Cre

Plasmid

#173616

Purpose

Expresses a Atrx-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Atrx (Atrx Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgAtrx#1/Cre

Plasmid

#173612

Purpose

Expresses a Atrx-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Atrx (Atrx Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hAP1 1-419

Plasmid

#183644

Purpose

To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Tags

myc

Expression

Mammalian

Mutation

truncated ATP6AP1 mutant; contains only amino aci…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hAP1 Δ420-440

Plasmid

#183642

Purpose

To (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Use

Lentiviral

Tags

HA

Mutation

truncated ATP6AP1 mutant lacking the transmembran…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hSEC61B-C-PEN2

Plasmid

#183648

Purpose

Lentiviral expression of a ER-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and SEC61B (PSENEN, SEC61B Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFbxw7#2/Cre

Plasmid

#173636

Purpose

Expresses a Fbxw7-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Fbxw7 (Fbxw7 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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