We narrowed to 15,772 results for: LEA

1. pGBW-m4133256

   Plasmid

   #152133

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHis-003

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available Since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. BtuCD

   Plasmid

   #92208

   Purpose

   Expression of BtuCD in E. coli

   Depositor

   Douglas Rees

   Article

   Locher et al Science. 2002 May 10;296(5570):1091-8.

   Inserts

   btuC (btuC )
   btuD (btuD )
   

   Tags

   10x Histidine Tag and Enterokinase Cleavage Site

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTB-Multibow-hG

   Plasmid

   #60994

   Purpose

   Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear EGFP

   Depositor

   Sean Megason

   Article

   Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

   Insert

   EGFP
   

   Use

   Zebrafish

   Tags

   histone 2B tag

   Promoter

   Bactin2

   Available Since

   Jan. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. 6CKO (DOT1L)

   Plasmid

   #119962

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   DOT1L (DOT1L Human)
   

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 1, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGBW-m4252890

   Plasmid

   #153809

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;V5

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGBW-m4046442

   Plasmid

   #145550

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pSaI20

   Plasmid

   #8615

   Depositor

   Robert Hartley

   Article

   Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

   Insert

   Ribonuclease inhibitor SaI20
   

   Expression

   Bacterial

   Mutation

   N terminal threnine changed to alanine

   Available Since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4046534

   Plasmid

   #145571

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF10 protein (ORF10 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGBW-m4046182

   Plasmid

   #149019

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. 5WBN (HDAC6)

    Plasmid

    #101267

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HDAC6 (HDAC6 Human)
    

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pGBW-m4133712

    Plasmid

    #152678

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;TapTag

    Expression

    Mammalian

    Mutation

    H. sapiens recode 1

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pGBW-m4133654

    Plasmid

    #152758

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;CBP

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4046372

    Plasmid

    #149048

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4046521

    Plasmid

    #145473

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046387

    Plasmid

    #149005

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046447

    Plasmid

    #145387

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4133590

    Plasmid

    #152377

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pST50Trc1-CBPDHFR

    Plasmid

    #63936

    Purpose

    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Calmodulin Binding Peptide (CBP)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBW-m4133578

    Plasmid

    #152329

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046526

    Plasmid

    #149101

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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