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pBB3-ScVip1-536-1107-R551A

Plasmid

#238320

Purpose

ScVip1-536-1107-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R551A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-E991A

Plasmid

#238321

Purpose

ScVip1-536-1107-E991A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E991A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H651A

Plasmid

#238323

Purpose

ScVip1-536-1107-H651A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H651A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 Rab34

Plasmid

#237338

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

Rab34 (RAB34 Mouse)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB160

Plasmid

#217841

Purpose

Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (L8K+Q71L) for pulldown assays

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf1 (ARF1 Human)

Tags

10x His, HaloTag, and SUMO (SMT3)

Expression

Bacterial

Mutation

L8K, Q71L

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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H75R SUMO1

Plasmid

#221060

Purpose

Expression of H75R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H75R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H75R

Promoter

T7

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FRB-Fis1 (no mt-mKeima)

Plasmid

#223795

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FRB-Fis1

Use

Lentiviral

Tags

FRB

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

Plasmid

#227009

Purpose

Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

Plasmid

#227010

Purpose

Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

Plasmid

#227011

Purpose

Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

Plasmid

#227012

Purpose

Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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GMR11F02-Gal4 UAS-GFP

Plasmid

#230907

Purpose

Tissue-specific expression of GFP in wing and haltere imaginal discs.

Depositor

Michael Boutros

Article

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

GFP

Expression

Insect

Promoter

GMR11F02Gal4-UAS

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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E67K SUMO1

Plasmid

#221065

Purpose

Expression of E67K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

E67K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

E67K

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35K SUMO1

Plasmid

#221055

Purpose

Expression of H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35K

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35S SUMO1

Plasmid

#221056

Purpose

Expression of H35S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35S site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35S

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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E67R SUMO1

Plasmid

#221064

Purpose

Expression of E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

E67R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

E67R

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H43R SUMO1

Plasmid

#221057

Purpose

Expression of H43R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H43R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H43R

Promoter

T7

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H35R SUMO1

Plasmid

#221054

Purpose

Expression of H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35R

Promoter

T7

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
H43K SUMO1

Plasmid

#221058

Purpose

Expression of H43K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H43K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H43K

Promoter

T7

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
H43S SUMO1

Plasmid

#221059

Purpose

Expression of H43S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H43S site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H43S

Promoter

T7

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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