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pCellFree_G03 GTF2H2D

Plasmid

#67081

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H2D (GTF2H2C_2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
TAL3144

Plasmid

#41318

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pum1-Left (pum1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3145

Plasmid

#41319

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pum1-Right (pum1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
PLEXIN A3

Plasmid

#58238

Purpose

Riboprobe for in vitro transcription of Plexin A3

Depositor

Christiana Ruhrberg

Article

Schwarz et al Dev Biol. 2008 Dec 1;324(1):1-9. doi: 10

Insert

Plexin A3 (Plxna3 Mouse)

Expression

Bacterial

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
TAL3200

Plasmid

#41374

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-vascular-endothelia-growth-factor-aa-(vegfaa)-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only
TAL3201

Plasmid

#41375

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-vascular-endothelia-growth-factor-aa-(vegfaa)-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

Plasmid

#101109

Purpose

Encodes dCas9(N)-DHFR fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)-DHFR

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pPI461

Plasmid

#113215

Purpose

generic knockin vector for C-terminal tagging with mCherry

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mCherry

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pPI457

Plasmid

#113200

Purpose

shuttle vector, C-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector, c-termin…

Tags

mScarlet

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPI418

Plasmid

#113199

Purpose

shuttle vector, N-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector, n-termin…

Tags

mScarlet

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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CD4_LD 0_TEV

Plasmid

#58870

Purpose

MESA protease chain with CD4 ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with CD4 ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pGEL089

Plasmid

#137897

Purpose

iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF10

Plasmid

#13109

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF10

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pJK_proK6_eGFP

Plasmid

#59850

Purpose

Constitutively expresses eGFP with proK6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK6

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pOVC1_L3(GSSSSGGSSSG)

Plasmid

#131210

Purpose

Cloning vector containing A-B-C casette with linker L3 in site B

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSSSGGSSSG llinker

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_L2(GSSGGSSG)

Plasmid

#131209

Purpose

Cloning vector containing A-B-C casette with linker L2 in site B

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSGGSSG linker

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_L1(GSSSG)

Plasmid

#131208

Purpose

Cloning vector containing A-B-C casette with linker L1 in site B

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSSG linker

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF64

Plasmid

#13200

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF64

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pJB073

Plasmid

#98912

Purpose

Express Dronpa tagged ZapB in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

ZapB

Tags

Dronpa

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAWp7-10

Plasmid

#72742

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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