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mCit(S1-S3)3-SAH

Plasmid

#228067

Purpose

Mammalian expression of coiled coil segments (S1-S3)x3, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCitrine, S1, S3

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2)3(S4)3-gs

Plasmid

#228068

Purpose

Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BORF2

Plasmid

#195834

Purpose

Gateway Entry Clone of Epstein-Barr virus BORF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BORF2

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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EYFP-NIK

Plasmid

#111208

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

NIK (MAP3K14 Human)

Tags

EYFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEasyG1_nat

Plasmid

#184911

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG3_mic

Plasmid

#184920

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA/Myc DeltaN TCF4

Plasmid

#16513

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Expression

Mammalian

Mutation

Delta N: lacks aa 1-32

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCDH_Str-KDEL_ManII-SBP-mCherry

Plasmid

#65259

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR223-BcLF1

Plasmid

#198581

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BcLF1

Use

Gateway Cloning

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BGLF1

Plasmid

#195818

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF1

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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