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Lenti-sgRNA-Cre-GpNLuc-sgmMLKL

Plasmid

#208391

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MLKL gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgMLKL (Mlkl Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BRD9(91))-PGKpuro2ABFP-W

Plasmid

#200474

Purpose

Lentiviral vector expressing gRNA targeting human BRD9

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

BRD9(91) (BRD9 Human)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

Plasmid

#209197

Purpose

Mutagenesis of Faah

Depositor

Larry Zweifel

Article

Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

Insert

Faah (Faah Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ICAM2-COMP5AP-AviTag-9xHis

Plasmid

#157174

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ICAM2 (ICAM2 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CEACAM18-Fc(DAPA)-AviTag-6xHis

Plasmid

#156797

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM18 (CEACAM18 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-R133C

Plasmid

#179409

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R133C)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(R133C) (Mecp2 Mouse)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R133C

Promoter

CAGGS

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-717pUC19-tNGFR-P2A-CD3E

Plasmid

#186111

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD3E (CD3E Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-745pUC19-tNGFR-P2A-CD4

Plasmid

#186115

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD4 (CD4 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-744pUC19-tNGFR-P2A-CD2

Plasmid

#186118

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD2 (CD2 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-716pUC19-tNGFR-P2A-CD3G

Plasmid

#186090

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD3G (CD3G Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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