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pAc5.1B-lambdaN-HA-DmCup_1-417-Y342AL347A-V5His6_M

Plasmid

#146901

Purpose

Insect Expression of DmCup_1-417-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_1-417-Y342AL347A (cup Fly)

Expression

Insect

Available Since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_10_0460_D463N

Plasmid

#171644

Purpose

Expression of L. major glcyoprotein-63 (D463N) with substrate binding site mutation from chromosome 10 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.10.0460 D463N

Tags

Myc-His

Expression

Mammalian

Mutation

D463N

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1-tTA/Zeo

Plasmid

#171685

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Zeocin

Expression

Mammalian

Promoter

RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDmHsp70b-TACT

Plasmid

#165780

Purpose

HSP70B promoter for fusion with bacterial promoters

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Hsp70b-TACT

Expression

Insect

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-CT-Tagging

Plasmid

#165871

Purpose

Template for amplification of EGFP C-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - CT Tagging

Use

Synthetic Biology

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_MAPRE1-Halo

Plasmid

#175345

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

MAPRE1 (MAPRE1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p3xP3

Plasmid

#165779

Purpose

3xP3 eye driver enhancer element, requires promoter element for function

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

3xP3

Expression

Insect

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCP6-STD

Plasmid

#165795

Purpose

Standard grammar bacterial CP6 promoter

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

CP6 promoter

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry TL1

Plasmid

#165832

Purpose

mCherry red fluorescent protein for N-terminal tagging

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

mCherry

Expression

Insect

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA43

Plasmid

#172944

Purpose

pQE30 encoding Wzz mutant i191

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i191 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-CT-Tagging

Plasmid

#165869

Purpose

Template for amplification of mcherry C-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - CT Tagging

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-NT-Tagging

Plasmid

#165868

Purpose

Template for amplification of EGFP N-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - NT Tagging

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFC31 attB O1

Plasmid

#165843

Purpose

_C31 integrase attB site in pColE1_Omega1

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 attB

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFC31 attB O2

Plasmid

#165844

Purpose

_C31 integrase attB site in pColE1_Omega2

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 attB

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSV40L-TN

Plasmid

#165827

Purpose

Viral Simian virus 40 Late poly(a) terminator element Compatible with transposon 3' ITRs

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

SV40L

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry TL5

Plasmid

#165833

Purpose

mCherry red fluorescent protein for C-terminal tagging

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

mCherry

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP TL1

Plasmid

#165834

Purpose

sfGFP green fluorescent protein for N-terminal tagging

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EGFP

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP TL5

Plasmid

#165835

Purpose

sfGFP green fluorescent protein for C-terminal tagging

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EGFP

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGGSx4 CT

Plasmid

#165811

Purpose

Flexible peptide linker of 4 repeats of GGS, for assembly of C-terminal TL5 tags to TL4 parts

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GGSx4 CT

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p4xLexAOp-A1

Plasmid

#165814

Purpose

Alpha1 4xLexAOp for assembly of larger LexAOp response elements

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

4xLexAOp-A1

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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