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pLU-CMV-Flag-hSesn2 T336A/F337A

Plasmid

#111787

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T336A/F337A

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 Q340A/D341A

Plasmid

#111788

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Q340A/D341A

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 C204S

Plasmid

#111782

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C204S

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pPLtetO-PdhR

Plasmid

#89655

Purpose

Plasmid for titrating synthesis of PdhR with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

Pyruvate dehydrogenase complex repressor

Use

Synthetic Biology

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pPLtetO-FtsB

Plasmid

#107559

Purpose

Plasmid for titrating synthesis of FtsB with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

ftsB

Use

Synthetic Biology

Promoter

pLtetO

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSMART-ala13

Plasmid

#87174

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proD

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

Plasmid

#70667

Purpose

Human mutant of E2F1 lacking amino acids 193-359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 193 to 359 (E2F1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

lacks amino acids 193-359

Promoter

cmv

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pDRf1-4CL5-AtSDT (JBEI-11535)

Plasmid

#87934

Purpose

Co-expression in S.cerevisiae of 4CL5 and AtSDT

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Inserts

SDT (SDT Mustard Weed)
4CL5 (4CL5 Mustard Weed)

Expression

Yeast

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-ala11

Plasmid

#87172

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proA

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-ala12

Plasmid

#87173

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proC

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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GIV-FLAG L249P (LP)

Plasmid

#86097

Purpose

Expression GIV in mammalian cell

Depositor

Pradipta Ghosh

Article

Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Mutation

L249P

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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const #7-RFP

Plasmid

#81081

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23109

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #6-RFP

Plasmid

#81080

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23117

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pWHI2-HYG

Plasmid

#80589

Purpose

CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

Depositor

John McCusker

Article

Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

Insert

WHI2 (WHI2 Budding Yeast)

Expression

Yeast

Promoter

WHI2 native promoter

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pET24_BD3179

Plasmid

#73142

Purpose

Expresses in E.coli B. bacteriovorus Bd3179

Depositor

Sandra Gabelli

Article

de la Peña et al PLoS One. 2015 Nov 2;10(11):e0141716. do

Insert

Bd3179

Expression

Bacterial

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pRFP shRNA-resistant TrkB F616A -S478A

Plasmid

#72718

Purpose

Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant and lacking TIAM1-binding site

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

TrkB

Tags

RFP

Expression

Mammalian

Mutation

shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
AEB-T4-2

Plasmid

#63855

Purpose

This pFTV1 vector contains a thyroxine binding riboswitch, controlling the translation initaition rate of luciferase gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

T4-2 thyroxine riboswitch

Use

Synthetic Biology

Promoter

tac

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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AEB-TMR7pUC19

Plasmid

#63857

Purpose

This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

TMR7 TMR riboswitch

Use

Synthetic Biology

Promoter

tac

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

Plasmid

#70669

Purpose

Human mutant of E2F1 lacking amino acids 284 to 359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 284 to 359 (E2F1 Human)

Tags

Flag

Expression

Mammalian

Mutation

lacks amin acids 284 to 359

Promoter

cmv

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only
MS4A1 RFP (805)

Plasmid

#62009

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

MS4A1

Tags

mRFP

Expression

Mammalian

Promoter

pgk

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only