We narrowed to 1,606 results for: Sp1

1. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

   Plasmid

   #169182

   Purpose

   Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp12-His6-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   His6-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

   Plasmid

   #193853

   Purpose

   Promoterless insertion vector for split HygR/mScarlet-I landing pad

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   5' rps-0::HygR + 5' mScarlet-I
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rps-0p

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

   Plasmid

   #205256

   Purpose

   S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP in pDBEcR-pIND(SP1)/Bla

   Plasmid

   #201557

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc and FLAG-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIMD(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

   Plasmid

   #201560

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIND(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223 SARS-CoV2 TEV-NSP16

   Plasmid

   #154414

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. EGFP in pDBEcR-pIND(SP1)-DD/Bla

   Plasmid

   #201559

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIND(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP in pDBEcR-pIND(SP1)-W/Bla

   Plasmid

   #201558

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc and FLAG-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIND(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223 SARS-CoV2 TEV-NSP14

   Plasmid

   #154412

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

    Plasmid

    #169189

    Purpose

    Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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