We narrowed to 7,149 results for: CIT

1. pK18sB

   Plasmid

   #177838

   Purpose

   Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

   Type

   Empty backbone

   Use

   Bacterial gene replacement

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-RFP

   Plasmid

   #22907

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Insert

   human Synapsin I promoter driving DsRedExpress (SYN1 Human)
   

   Use

   AAV; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-hSyn-RFP

   Plasmid

   #22909

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Has Service

   Cloning Grade DNA

   Insert

   human synapsin I promoter driving DsRedExpress (SYN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Cre

   Plasmid

   #123133

   Purpose

   Mammalian expression vector for wild-type Cre.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Cre recombinase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRT/Flag-LOC339287(MSL1)

   Plasmid

   #29762

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   MSL1 (MSL1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5FRT/Flag-MSL3L1

   Plasmid

   #29761

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   MSL3L1 (MSL3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2013

   Availability

   Academic Institutions and Nonprofits only

7. CaMKIIP-mEGFP-P2A-paAIP2/pAAV

   Plasmid

   #91718

   Purpose

   AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

   Insert

   mEGFP-P2A-paAIP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII 1.3

   Available since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL-his-LbCpf1-EC

   Plasmid

   #79008

   Purpose

   Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Insert

   LbCpf1
   

   Use

   Tags

   MBP-6xHis-NLS

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG mito-GCaMP5G

   Plasmid

   #105009

   Purpose

   mitochondrial calcium sensor

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   GCaMP5G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTagRFP657-C1

    Plasmid

    #31872

    Purpose

    For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

    Depositor

    Vladislav Verkhusha

    Article

    Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

    Insert

    TagRFP657
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. SlugCas9

    Plasmid

    #163793

    Purpose

    Expresses SlugCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FokI-dCas9

    Plasmid

    #52970

    Purpose

    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

    Depositor

    David Liu

    Article

    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Fok1 fused to dCas9
    

    Use

    CRISPR

    Tags

    Fok1 nuclease (homodimeric wildtype) and NLS

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-syn-ChR2-EYFP-Kv

    Plasmid

    #89256

    Purpose

    Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Insert

    Channelrhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET20b-delta133HtrA2

    Plasmid

    #14126

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-2

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. miReporter-CAG

    Plasmid

    #82478

    Purpose

    microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. miReporter-PGK

    Plasmid

    #82477

    Purpose

    microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. xCas9 3.7

    Plasmid

    #108379

    Purpose

    Mammalian expression vector for xCas9 3.7

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9 3.7
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. His6-TEV-Venus-pBluescript

    Plasmid

    #166756

    Purpose

    For purification of Venus fluorophore

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Venus
    

    Use

    Tags

    6x Histidine tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-Bxb1-His

    Plasmid

    #123132

    Purpose

    Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Bxb1 integrase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJJB1561

    Plasmid

    #218626

    Purpose

    Express geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimer

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    GES
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-Ef1a-fDIO-eGFP-WPRE

    Plasmid

    #203843

    Purpose

    Flp-dependent EGFP expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCS Omomyc

    Plasmid

    #113164

    Purpose

    Omomyc expression in mammalian cells

    Depositor

    Sergio Nasi

    Article

    Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

    Insert

    Omomyc (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E57T, E64I, R70Q, R71N

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLenti6.3-enCjCas9-BlastR

    Plasmid

    #220978

    Purpose

    Expresses human codon-optimized enCjCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    enCjCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCZGY847

    Plasmid

    #135093

    Purpose

    Transcriptional mcherry reporter of acr-2 (cholinergic) expressing neurons in C. elegans

    Depositor

    Yishi Jin

    Article

    Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

    Insert

    Pacr-2
    

    Use

    Tags

    mcherry and unc-10 3'UTR

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. PdnaK GFP pZa

    Plasmid

    #170073

    Purpose

    GFP expression under the control of E. coli dnaK promoter

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PdnaK

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET-His-Cre

    Plasmid

    #123128

    Purpose

    Bacterial expression plasmid for wild-type Cre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pFCcGi

    Plasmid

    #59324

    Purpose

    Inducible expression of GFP and constitutive expression of mCherry

    Depositor

    David Holden, Sophie Helaine

    Article

    Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

    Inserts

    mCherry
    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and rpsM

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BiP 2: C. elegans BiP (hsp-4)-GFP

    Plasmid

    #21896

    Depositor

    David Ron

    Article

    Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

    Insert

    BiP (hsp-4 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Fusion between the GFP and hsp-4 takes place at A…

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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29. pmCherry_gRNA

    Plasmid

    #80457

    Purpose

    gRNA expression vector with mCherry transfection control

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Inserts

    sp Cas9 gRNA
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin promoter

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GAL4-DrBphP

    Plasmid

    #158986

    Purpose

    Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cells

    Depositor

    Liangcai Gu

    Article

    Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

    Insert

    GAL4-DrBphP
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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