We narrowed to 13,583 results for: TIM

1. SJZ7-KAN

   Plasmid

   #228260

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   kan
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPM1 S.c.

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SJZ4-NAT

   Plasmid

   #228257

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   nat1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   FBA1 S.c.

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cdk1-mCherry

   Plasmid

   #54466

   Purpose

   CDK1-mCherry in pIVT vector for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

   Insert

   Cdk1 mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMAL-BRT17

   Plasmid

   #45358

   Depositor

   Scott Banta

   Article

   Shur et al Biotechniques. 2013 Apr;54(4):197-206. d

   Insert

   17 Repeat Beta Roll Tag
   

   Use

   Tags

   Maltose Binding Protein (MBP)

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet

   Plasmid

   #130509

   Purpose

   expresses mScarlet

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry2-mEGFP

   Plasmid

   #127814

   Purpose

   Hetero-dimer mCherry2-mEGFP expression vector

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   mCherry2-mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRec ΔLacI

   Plasmid

   #104575

   Purpose

   Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.

   Depositor

   Harris Wang

   Article

   Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

   Insert

   PTet-cas12, tetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEB2-2ndVal mCherry2-L

   Plasmid

   #104027

   Purpose

   Plasmid encoding 2ndVal mCherry2-L

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   2ndVal mCherry2-L
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   mCherry2-L with valine inserted in 2nd position

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-EPN-01(HiBiT)

   Plasmid

   #162590

   Purpose

   EPN-01 tagged with HiBiT tag under CMV promoter

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

   Insert

   EPN-01
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX4T-3-6AOptParkin

    Plasmid

    #165107

    Purpose

    bacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin gene

    Depositor

    Arthur Haas

    Insert

    Parkin isoform 1 (PRKN Human)
    

    Use

    Tags

    GST-6-alanine

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFH47

    Plasmid

    #125812

    Purpose

    Level0 LbCas12a, human codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    LbCas12a, human codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-51b-HaloTag11

    Plasmid

    #175519

    Purpose

    Expression of HaloTag11 in bacterial cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag11
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEB1-mGFPmut3

    Plasmid

    #103981

    Purpose

    Plasmid encoding mGFPmut3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mGFPmut3
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65G, S72A, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEB2-2ndVal mScarlet-I

    Plasmid

    #104028

    Purpose

    Plasmid encoding 2ndVal mScarlet-I

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    2ndVal mScarlet-I
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    mScarlet-I with valine inserted in 2nd position

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRec

    Plasmid

    #104572

    Purpose

    Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.

    Depositor

    Harris Wang

    Article

    Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

    Insert

    PTet-cas12, tetR, lacI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CS2 Flag-Smad3

    Plasmid

    #14052

    Depositor

    Joan Massague

    Article

    Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pEB2-2ndVal Katushka

    Plasmid

    #104029

    Purpose

    Plasmid encoding 2ndVal Katushka

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    2ndVal Katushka
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Katushka with valine inserted in 2nd position

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEB1-Sapphire

    Plasmid

    #103976

    Purpose

    Plasmid encoding Sapphire

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    Sapphire
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S72A, Y145F,…

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDS1902

    Plasmid

    #167278

    Purpose

    Expression plasmid for Candida albicans containing a red-shifted firefly luciferase

    Depositor

    Dominique Sanglard

    Article

    Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

    Insert

    red shifted luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    ACT1

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEB2-2ndVal mKate2

    Plasmid

    #104030

    Purpose

    Plasmid encoding 2ndVal mKate2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    2ndVal mKate2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    mKate2 with valine inserted in 2nd position

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDRESS_mTurquoise2_spatial-linker-P2A_mScarletI

    Plasmid

    #130510

    Purpose

    expresses mScarlet1

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

    Insert

    mScarlet1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 4xmEGFP

    Plasmid

    #127801

    Purpose

    Homo-tetramer mEGFP-mEGFP-mEGFP-mEGFP expression vector

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    mEGFP-mEGFP-mEGFP-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEB1-mEYFP

    Plasmid

    #103985

    Purpose

    Plasmid encoding mEYFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mEYFP
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65G, V68L, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFH13

    Plasmid

    #125798

    Purpose

    Level0 SpCas9, wheat codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    SpCas9, wheat codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TroponinT-Gateway

    Plasmid

    #46026

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    TNNT2

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-VSV-G-HiBiT

    Plasmid

    #162594

    Purpose

    C-terminally HiBiT-tagged VSV-G under CMV promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    VSV-G
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFH17

    Plasmid

    #125803

    Purpose

    Level0 LbCas12a, rice codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    LbCas12a, rice codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYES2.1-pomMet1

    Plasmid

    #107444

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met1
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEB2-TurboRFP

    Plasmid

    #103996

    Purpose

    Plasmid encoding TurboRFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TurboRFP
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, T68A, L79F, …

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

    Plasmid

    #169326

    Purpose

    Expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge