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pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP

Plasmid

#188272

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b(Q119F/E475R/E758R)

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-mut2_F

Plasmid

#146272

Purpose

Insect Expression of DmMe31B-mut2

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmMe31B-mut2 (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and one non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-R72K

Plasmid

#127683

Purpose

Bacterial expression of His-tagged OphA-delta C6-R72K

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-R72K

Tags

His8

Expression

Bacterial

Mutation

delta C6-R72K

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C18

Plasmid

#127678

Purpose

Bacterial expression of His-tagged OphA-delta C18

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C18

Tags

His8

Expression

Bacterial

Mutation

delta C18

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y63F

Plasmid

#127680

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y63F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y63F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y63F

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y66F

Plasmid

#127681

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y66F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y66F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y66F

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-R72A

Plasmid

#127682

Purpose

Bacterial expression of His-tagged OphA-delta C6-R72A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-R72A

Tags

His8

Expression

Bacterial

Mutation

delta C6-R72A

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y76F

Plasmid

#127684

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y76F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y76F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y76F

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OpHA-delta C6-Y98A

Plasmid

#127685

Purpose

Bacterial expression of His-tagged OpHA-delta C6-Y98A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OpHA-delta C6-Y98A

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y98A

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y98F

Plasmid

#127686

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y98F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y98F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y98F

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Q172A

Plasmid

#127687

Purpose

Bacterial expression of His-tagged OphA-delta C6-Q172A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Q172A

Tags

His8

Expression

Bacterial

Mutation

delta C6-Q172A

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-W400A

Plasmid

#127688

Purpose

Bacterial expression of His-tagged OphA-delta C6-W400A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-W400A

Tags

His8

Expression

Bacterial

Mutation

delta C6-W400A

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-P399A

Plasmid

#127689

Purpose

Bacterial expression of His-tagged OphA-delta C6-P399A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-P399A

Tags

His8

Expression

Bacterial

Mutation

delta C6-P399A

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(I325A)

Plasmid

#122272

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-E78A/E83A

Plasmid

#100272

Purpose

Expresses GST-Stx3-E78A/E83A in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-E78A/E83A (Stx3 Rat)

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 gnu

Plasmid

#112999

Purpose

For protein expression and purification of full-length Drosophila gnu

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length gnu (gnu Fly)

Expression

Bacterial

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D19-2/5

Plasmid

#91272

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-2/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-2/5 (SH3D19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_HBP1

Plasmid

#86272

Purpose

Donor vector for 3' FLAG tag of human HBP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

HBP1 homology arms (HBP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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TAL3098

Plasmid

#41272

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-hsd17b4-Left (hsd17b4 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00621

Plasmid

#39693

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG31392 (CG42726 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only