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pAAV-syn-dLight1.3b

Plasmid

#135762

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-dLight1.2

Plasmid

#111068

Purpose

To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1 and AAV5

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL11056

Plasmid

#68258

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_AchLightG

Plasmid

#187464

Purpose

Expresses green acetylcholine indicator AchLightG in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AchLightG

Use

AAV

Tags

Igκ leader

Expression

Mutation

Promoter

human Synapsin-1

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_AchLightR

Plasmid

#187463

Purpose

Expresses red acetylcholine indicator AchLightR in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AchLightR

Use

AAV

Tags

Igκ leader

Expression

Mutation

Promoter

human Synapsin-1

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL11055

Plasmid

#68252

Purpose

Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.2

Plasmid

#111069

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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R

Plasmid

#167170

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R
ATP1B1

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

P10 and PH

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2ABFP

Plasmid

#136405

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

BbsI restriction site at position 437 was removed…

Promoter

U6, PDK

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190076

Purpose

express human ACE2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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S

Plasmid

#167172

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-S
ATP1B1

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

P10 and PH

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_AdoLightG

Plasmid

#187465

Purpose

Expresses green adenosine indicator AdoLightG in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AdoLightG

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

human Synapsin-1

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190078

Purpose

express Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (TMPRSS2 Rhinolophus ferrumequinum)

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[HA]_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190080

Purpose

express cytoplasmically HA-tagged human ACE2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSHDY*in_PpetE:BCD2:MrBBS:Strep

Plasmid

#133973

Purpose

Heterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.

Depositor

Pia Lindberg

Article

Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

Insert

MrBBS:StrepII

Use

Synthetic Biology

Tags

StrepII

Expression

Bacterial

Mutation

inserted gene is codon optimized for Synechocyst…

Promoter

PpetE:BCD2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.3b

Plasmid

#125560

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1, AAV5, and AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171594

Purpose

Strong human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (mPCSK9)

Plasmid

#170122

Purpose

AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (Pcsk9 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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LentiRCas9-CUG

Plasmid

#104183

Purpose

Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

Depositor

Eugene Yeo

Article

Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

Insert

U6-CUGsgRNA, EFS-PIN-dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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