We narrowed to 3,142 results for: BFP

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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iC30 PB-Zim3-dCas9-P2A-BFP_hygro

Plasmid

#236743

Purpose

Constitutive expression of Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI)

Inserts

CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
EF1a-hygro-WPRE

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-LTR-dCas9-VP64-BFP

Plasmid

#46912

Purpose

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

dCas9-VP64-BFP fusion
Puromycin resistance

Use

CRISPR and Retroviral

Tags

3xNLS, BFP, and VP64 domain

Expression

Mammalian

Promoter

LTR and PGK

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a-P2A-tagBFP

Plasmid

#84569

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-sgRNAs-del-ZNF91-BFP

Plasmid

#202823

Purpose

Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and BFP expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

two sgRNAs (each with a U6 promoter) to delete exon 1 within ZNF91 gene

Use

Lentiviral

Expression

Mammalian

Promoter

U6 - two

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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hGFAP-NLS-HA-Dre-P2A-BFP

Plasmid

#51277

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Dre-P2A-BFP

Expression

Mammalian

Promoter

hGFAP

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

Plasmid

#154139

Purpose

Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP

Tags

3x FLAG and tagBFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAF0689 Ef1a-Pa01_attB-BFP acceptor

Plasmid

#193472

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attB

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo (Universal)

Plasmid

#80767

Purpose

Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_UAS-tTS-P2A-GFP_PGK-BFP

Plasmid

#133808

Purpose

inducible expression of tTS and GFP with BFP marker in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

tTS-P2A-EGFP-PGK-BFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT1

Plasmid

#66818

Purpose

dCas9 fused to BFP and the human DNMT1 catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dCas9-BFP-DNMT1, catalytic domain (DNMT1 )

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-BFP

Plasmid

#85449

Purpose

Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-BFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHR Ef1a-dCas9-BFP-KRAB

Plasmid

#217304

Purpose

lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

Depositor

Luke Gilbert

Insert

dCas9-tagBFP-KRAB

Use

CRISPR, Lentiviral, and Synthetic Biology

Promoter

Ef1alpha

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-EBFP2-NLS (pRW1447)

Plasmid

#216219

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-iCre-ERT2

Plasmid

#128168

Purpose

Monomeric TagBFP2 and iCre-ERT2 recombinase (PAPGSTMA N-terminus)

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Plasmid

#64512

Purpose

Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XmTagBFP2

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pPD687 A2UCOE-HBB_IVS2-EnAsCas12a-BFP

Plasmid

#249160

Purpose

Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

A2UCOE-HBB_IVS2-EnAsCas12a-TagBFP (HBB Synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

HBB IVS2 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19/PLIN2 GFP-P2A-BFP

Plasmid

#202195

Purpose

knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

Cloning vector

Tags

GFP-P2A-BFP knock-in

Promoter

lac

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M

Plasmid

#102591

Purpose

Lentivector to express STING V155M and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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