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pAGM62624

Plasmid

#169094

Purpose

Low copy MoClo level 2 binary vector for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM62636

Plasmid

#169095

Purpose

Low copy MoClo level P binary vector for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PPP1CB-miRFP703

Plasmid

#178524

Purpose

Expresses PP1c-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

PP1c-miRFP703 (PPP1CB Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBSFI-p110γ

Plasmid

#34892

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

Insert

p110γ (PIK3CG Human)

Use

Adaptor plasmid

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-HA_N-human Prkd2

Plasmid

#137850

Purpose

Express Prkd2 in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

Protein kinase d2 (PRKD2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAGM53151

Plasmid

#169093

Purpose

Level 1 cloning vector for transient expression. Already contains the 35S promoter and the Nos terminator.

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM24951

Plasmid

#169088

Purpose

MoClo level 0 Tomato E8 promoter module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Tomato E8 promoter

Use

Synthetic Biology; Moclo cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM921

Plasmid

#169086

Purpose

MoClo level 0 Db5GT coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Db5GT

Use

Synthetic Biology; Moclo cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1 GBP (GBP-mCherry)

Plasmid

#162879

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJS699

Plasmid

#168779

Purpose

Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Insert

Spike RBD (S betacoronavirus)

Use

Tags

Cmyc

Expression

Mutation

N343Q

Promoter

GAL1

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pBSFI-p110δ

Plasmid

#34893

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

Insert

p110δ (PIK3CD Human)

Use

Adaptor plasmid

Tags

Expression

Mutation

See depositor comments below.

Promoter

Available since

Feb. 23, 2012

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Ectodomain M400C

Plasmid

#159642

Purpose

Contains integrin alpha v residues 1–960 with the M400C mutation

Depositor

Timothy Springer

Article

Wang et al Proc Natl Acad Sci U S A. 2017 May 23;11

Insert

Integrin alpha v ectodomain

Use

Tags

Expression

Mammalian

Mutation

M400C

Promoter

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM7547

Plasmid

#169091

Purpose

MoClo level 1 35S promoter-Db5GT-35S terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-Db5GT-35S terminator

Use

Moclo cloning vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBSR (mCherry-surrogateTarget-bsr)

Plasmid

#66950

Purpose

Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

Depositor

Silvestro Conticello

Article

Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

Insert

blasticidin

Use

CRISPR, Mouse Targeting, and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD2-ODPBMTS (12)

Plasmid

#101695

Purpose

ODPBMTS in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from S. cerevisiae Pyruvate dehydrogenase E1 component subunit beta (YBR221C)]-AATG

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Gucy1b3

Plasmid

#105458

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Gucy1b3 (Gucy1b1 Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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TC1154

Plasmid

#164876

Purpose

Split GFP reporter plasmid expressing GFP (b11beta

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

Split GFP(beta11)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-mouse Bcl6

Plasmid

#138410

Purpose

Express mouse Bcl6 in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

B cell lymphoma 6 (Bcl6 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-human Bcl6

Plasmid

#137851

Purpose

Express Bcl6 in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

B cell lymphoma 6 (BCL6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-human Prkd2 without kinase domain

Plasmid

#138411

Purpose

Express Prkd2 without kinase domain in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

Prkd2 without kinase domain (PRKD2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

kinase domain deleted from human Prkd2

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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