We narrowed to 1,270 results for: grna cloning vector

1. pMXS-IRES-Blast ACSL3

   Plasmid

   #173165

   Purpose

   Retroviral vector to express sgRNA resistant human ACSL3

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Acyl-CoA Synthetase Long Chain Family Member 3 (ACSL3 Human)
   

   Use

   Retroviral

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available Since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-SauriCas9-KKH-Puro

   Plasmid

   #135966

   Purpose

   Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-hIP-dCas9-BSD

   Plasmid

   #183231

   Purpose

   Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   Human insulin promoter

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SAM-DNMT3A empty

   Plasmid

   #213164

   Purpose

   Empty vector (no sgRNA) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SAM-DNMT3A-inactiveEmpty

   Plasmid

   #213168

   Purpose

   Empty Vector (no sgRNA) used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

   Plasmid

   #190898

   Purpose

   Entry vector for multiple sgRNA cloning

   Depositor

   Nicole Déglon

   Article

   Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6 and 7sk

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYJA5

   Plasmid

   #217778

   Purpose

   The empty vector for quadruple sgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Puromycin resistance gene, T2A, and TagBFP

   Expression

   Mammalian

   Promoter

   PGK, CMV, U6

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-PGK-DIO-mCherry-TLR4miR-TLR2miR

   Plasmid

   #137733

   Purpose

   Lentivirus vector for TLR2/4 miR in a Cre-dependent manner

   Depositor

   Tomoyuki Furuyashiki

   Article

   Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

   Insert

   TKR2/4 microRNA
   

   Use

   Lentiviral

   Tags

   mChherry

   Expression

   Mammalian

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2

   Plasmid

   #204852

   Purpose

   AAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouse

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd6-nSaCas9KKH(D10A)-UGI
   

   Use

   AAV and CRISPR

   Mutation

   D10A, E782K, N968K, R1015H in SaCas9

   Promoter

   EFS, miniU6

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid

    #170185

    Purpose

    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Inserts

    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CBh and hU6

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry

    Plasmid

    #170184

    Purpose

    Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Inserts

    Sa163 RT
    Retron Sa163 msr-msd
    BFP-to-GFP conversion donor template An
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CBh and hU6

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #135012

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Tags

    3x Flag, 3xFLAG (N terminal on insert), NLS, and …

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXS-IRES-Blast HA-GPAT4-ΔNs5

    Plasmid

    #173169

    Purpose

    Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    Synonymous mutations at sgRNA sites, amino acids …

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

    Plasmid

    #173167

    Purpose

    Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
    

    Use

    Retroviral

    Tags

    3XFLAG

    Mutation

    Synonymous mutations at sgRNA sites, G2A, S6A

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO_AU1.C.HA_mCherry-NLS

    Plasmid

    #178209

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.HA and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO_Ollas.S.V5_mCherry-NLS

    Plasmid

    #178211

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    Nuclear Localization Signal and Ollas.S.V5

    Promoter

    eF1a

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO_AU1.HSV.S_mCherry-NLS

    Plasmid

    #178224

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HSV.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO_HA.NWS.Ollas_mCherry-NLS

    Plasmid

    #178263

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.NWS.Ollas and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_NWS.HA.V5_mCherry-NLS

    Plasmid

    #178283

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    NWS.HA.V5 and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_AU1.VSVg.V5_mCherry-NLS

    Plasmid

    #178236

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.VSVg.V5 and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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