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NK617

Plasmid

#109095

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

S. cerevisiae CoxIV target sequence

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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NK624

Plasmid

#109094

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

HDEL peptide

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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BB2_L_EF_syn_BbsI

Plasmid

#89921

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSE and FSF (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_HI_syn_BbsI

Plasmid

#89924

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSH and FSI (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB1_T_34_ani_trpC

Plasmid

#90291

Purpose

BB1 terminator trpC gene Aspergillus nidulans between FS3 and FS4

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

trpC

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB1_L_12_syn_BsaI

Plasmid

#89914

Purpose

Linker to construct BB1 from PCR product with FS1 and FS2 (in with BsaI, out with BbsI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB1_L_34_syn_BsaI

Plasmid

#89916

Purpose

Linker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_AB_syn_BbsI

Plasmid

#89917

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSA and FSB (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_BC_syn_BbsI

Plasmid

#89918

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSB and FSC (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_CD_syn_BbsI

Plasmid

#89919

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSC and FSD (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_DE_syn_BbsI

Plasmid

#89920

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_FG_syn_BbsI

Plasmid

#89922

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_GH_syn_BbsI

Plasmid

#89923

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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luxB:cp157Venus+luxA/pRSETB Duet

Plasmid

#197583

Purpose

BRET lux luciferase with cp157Venus for bacterial expression

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxBcp157Venus

Use

E.coli expression

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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GW1-ARSeNL

Plasmid

#207873

Purpose

Mammalian expression of the BRET ATP sensor

Depositor

Mathew Tantama

Article

Min et al Sensors (Basel). 2019 Aug 10;19(16). pii

Insert

ARSeNL

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEM790

Plasmid

#242607

Purpose

T-DNA plasmid, Level 2 destination vector, replaces pMIN-VS1

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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p5xATF6-GL3

Plasmid

#11976

Purpose

Reporter gene with five ATF6 sites that is highly sensitive to ER stress

Depositor

Ron Prywes

Article

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

5xATF6 binding site

Tags

Luc and c-fos promoter

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pET29-neoLux1.2

Plasmid

#229952

Purpose

Bacterial expression for de novo luciferase, neoLux1.2

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

neoLux1.2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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GCEPIA1-SNAPER

Plasmid

#172659

Purpose

Detect the real-time ER calcium store and local calcium microdomains in live cells.

Depositor

Pingyong Xu

Article

Luo et al Biophys Rep (2019) 5, 31–42

Insert

calmodulin

Expression

Mammalian

Mutation

Please see depositor comments

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shNT

Plasmid

#109012

Purpose

short-hairpin knockdown of targeted gene

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting shRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6 promoter

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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