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pHM129

Plasmid

#225534

Purpose

Encodes destablized mCherry under AR8 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

mCherry
BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFPV25.1

Plasmid

#20668

Purpose

S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Use

Tags

GFP mut3

Expression

Bacterial

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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Human-Dysferlin-Original

Plasmid

#60216

Purpose

Human dysferlin (accession #NM_003494) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.

Depositor

Jain Foundation

Insert

Dysferlin (DYSF Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pwB2b

Plasmid

#32094

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Inserts

BKV VP1
BKV VP2

Use

Tags

Expression

Mammalian

Mutation

codon modified

Promoter

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pET28CLY3

Plasmid

#21762

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pCAG/HA-hBIG1

Plasmid

#226253

Purpose

Expresses N-terminally HA-tagged hBIG1 in mammalian cells

Depositor

Hye-Won Shin

Insert

BIG1 (ARFGEF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHM131

Plasmid

#225530

Purpose

Encodes destablized mCherry under IBRE4 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

mCherry
BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET28CLY2

Plasmid

#21761

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing two SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2009

Availability

Academic Institutions and Nonprofits only
pPICZalphaB-SapL3

Plasmid

#78171

Purpose

Expression of Saporin L3 in pichia

Depositor

Vern Schramm

Article

Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

Insert

Saporin L3

Use

Tags

alpha factor

Expression

Yeast

Mutation

Promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBB

Plasmid

#53210

Purpose

Contains a 103bp telomeric track

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TTAGGG(n) Track

Use

Tags

Expression

Mutation

Promoter

T7

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3

Plasmid

#203460

Purpose

jAspSnFR3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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p7XNH3

Plasmid

#47064

Purpose

FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pME eos

Plasmid

#73792

Purpose

Photoconvertible eos in a middle entry clone

Depositor

David Raible

Article

Prendergast et al Development. 2012 Mar;139(6):1141-52. do

Insert

EOS

Use

Middle entry clone

Tags

Expression

Mutation

Promoter

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET28CLY9

Plasmid

#21769

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pYZ183

Plasmid

#98425

Purpose

Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ184

Plasmid

#98426

Purpose

Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTK222

Plasmid

#13532

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Use

Tags

See map

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pSYFP2-SCFP3A

Plasmid

#22905

Depositor

Dorus Gadella

Article

Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

Insert

SYFP2-SCFP3A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pET28CLY5

Plasmid

#21764

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 5 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pMOTag-glmS-4H

Plasmid

#106378

Purpose

Can be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS-3xHA

Use

Tags

Expression

Mutation

Promoter

Available since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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