19,834 results

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plenti-UbcP-HA-CRBN-pGK-HYG

Plasmid

#107378

Purpose

Lentiviral expression of HA-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UbcP

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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VPS29-YFP

Plasmid

#174520

Purpose

Expresses human VPS29 with a C-terminal YFP in mammalian cells

Depositor

Juan Bonifacino

Article

Rojas et al J Cell Biol. 2008 Nov 3;183(3):513-26. d

Insert

VPS29 (VPS29 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pF CAG luc-EGFP-cre puro

Plasmid

#67503

Purpose

Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

luciferase-EGFP-cre

Use

Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE

Plasmid

#162382

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

CAG

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-pcDNA3.1-

Plasmid

#52534

Purpose

enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Type

Empty backbone

Tags

RGS-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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L-HBsAg

Plasmid

#103011

Purpose

Expresses HBV Large envelope glycoprotein in mammalian cells

Depositor

Wang-Shick Ryu

Article

Wang et al PLoS Pathog. 2010 Jul 15;6(7):e1000986.

Insert

HBV Large envelope protein

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TATV) (pY220)

Plasmid

#89353

Purpose

Expresses humanized AsCpf1 variant that recognizes TATV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RVR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K548V/N552R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-R26-FLEX-mStr

Plasmid

#135619

Purpose

Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

Depositor

Lin He

Article

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

CAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAG425GPD-ccdB

Plasmid

#14154

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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FusionRed-Fascin-C-10

Plasmid

#56117

Purpose

Localization: Cytoskeleton, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Fascin (FSCN1 Human)

Tags

FusionRed

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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LeGO-mOrange2

Plasmid

#85212

Purpose

Lentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoter

Depositor

Boris Fehse

Article

Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

Insert

mOrange2

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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p5sheLL

Plasmid

#46953

Purpose

encode HPV5 L1 and L2, Bicistronic, too large to self-package

Depositor

John Schiller

Article

Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

Insert

HPV5L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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hNET pcDNA3

Plasmid

#15475

Depositor

Randy Blakely

Article

Galli et al J Exp Biol. 1995 Oct . 198(Pt 10):2197-2

Insert

hNET (SLC6A2 Human)

Expression

Mammalian

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV6-AC 4-μHOM

Plasmid

#113619

Purpose

Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

4-μHOM variant of EGFP

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pScCas9

Plasmid

#117700

Purpose

Mammalian expression vector encoding WT ScCas9

Depositor

Joseph Jacobson

Article

Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

Insert

Streptococcus canis Cas9

Use

CRISPR

Expression

Mammalian

Promoter

EF-1α

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry- eMagBF - 5ptaseOCRL

Plasmid

#162254

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

mCherry- eMagBF - 5ptaseOCRL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pflareG-psd95-exon18

Plasmid

#90266

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Insert

Psd95-exon18 (Dlg4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBJ FLAG-hPar1

Plasmid

#53226

Purpose

mammalian expression of human Par1 with N-terminal FLAG tag

Depositor

Shaun Coughlin

Article

Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

Insert

Par1 (F2R Human)

Tags

FLAG and bovine prolactin signal sequence (MDSKGS…

Expression

Mammalian

Mutation

Deletion of aa1-39 (native signal peptide)

Promoter

SV40

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-MET

Plasmid

#23889

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MET (MET Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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