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p15aC-4D1D

Plasmid

#107864

Purpose

Bacterial expression of PIS and PI4K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-kinase β

Tags

myc

Expression

Bacterial

Promoter

proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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Ad-EphA3 KD

Plasmid

#102726

Purpose

Expression of Kinase dead EphA3

Depositor

Jin Chen

Article

Frieden et al Dev Dyn. 2010 Dec;239(12):3226-34. doi:

Insert

EphA3 (Epha3 Mouse)

Use

Adenoviral

Mutation

Kinase Dead (K657M; AAA > ATG)

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express

Plasmid

#103995

Purpose

Plasmid encoding DsRed-Express

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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SH4-FRB-mRFP

Plasmid

#100741

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

Fyn

Tags

FRB and mRFP

Expression

Mammalian

Mutation

SH4 domain only

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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GST-beta2(616-951)

Plasmid

#100743

Purpose

Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

GST

Expression

Bacterial

Mutation

Long isoform

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

Plasmid

#89872

Purpose

expresses CB1R-iTango component in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

CB1R-V2tail-TevN-BLITz1-TetR-VP16

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSico-shNKCC1

Plasmid

#83027

Purpose

expresses an shRNA against NKCC1

Depositor

Angelique Bordey

Article

Young et al J Neurosci. 2012 Sep 26;32(39):13630-8.

Insert

shNKCC1

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpe

Plasmid

#79076

Purpose

Expression of mouse Cebpe

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp epsilon (Cebpe Mouse)

Use

Lentiviral

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
GlycophorinC-CD4d3+4-bio

Plasmid

#73096

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinC with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Glycophorin C (GYPC Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1 S222TAG

Plasmid

#68302

Purpose

N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 S222TAG (MAP2K1 Synthetic, Human)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-ER-E2F2-dRb

Plasmid

#62805

Purpose

expresses an inducible E2F2 mutant that lacks the Rb binding domain

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

E2F2 (E2F2 Human)

Use

Retroviral

Tags

HA-Er

Expression

Mammalian

Mutation

deletion of amino acids 302-437, including the Rb…

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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miniSOG-Alpha-Actinin-19

Plasmid

#57762

Purpose

Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Alpha-Actinin (ACTN1 Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pBacPAK p110a RBD DM

Plasmid

#55723

Purpose

His3 GST tagged RBD double mutant p110 alpha T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

His3 GST

Mutation

T207D, K227A

Promoter

polyhedrin promoter

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-hs-CDC34

Plasmid

#52731

Purpose

retroviral expression of human CDC34

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

CDC34 (CDC34 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-ERK5 K84R

Plasmid

#53171

Purpose

Expresses GST-HA tagged human ERK5 K84R from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

K84R

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 FL

Plasmid

#47090

Depositor

Marina Ramirez-Alvarado

Article

Martin et al Amyloid. 2010 Sep;17(3-4):129-36. doi: 1

Insert

AL-103 (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pIhh-5A9-LUC

Plasmid

#46318

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

5 copies of A9 sequence from Ihh promoter

Tags

luciferase

Expression

Mammalian

Promoter

mouse osteocalcin gene 2 promoter

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 epsilon

Plasmid

#44936

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 epsilon (GRF10 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET21-E. coli Initiation Factor 3 (IF3)

Plasmid

#31340

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

E. coli Initiation Factor 3

Tags

His

Expression

Bacterial

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pRS416 Gal TDP43 M337V

Plasmid

#27460

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Mutation

Methionine 337 mutated to Valine (M337V)

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only