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We narrowed to 16,183 results for: nes

Showing: 10621 - 10640 of 16183 results
  1. pJET-07-mNeonGreen-R11a

    Plasmid
    #179890
    Purpose
    Donor with homologous arms for Rab11a to knock in mNeonGreen
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB11A, member RAS oncogene family (RAB11A Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    mNeonGreen
    Expression
    Bacterial and Mammalian
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. PB-PE VQR

    Plasmid
    #162797
    Purpose
    All-in-one prime editor piggyBac transposon, VQR variant
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pRC/CMV-TYK21054-5FF-VSV

    Plasmid
    #139347
    Purpose
    Expression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loop
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    Expression
    Mammalian
    Mutation
    V362F-Y1054F-Y1055F
    Promoter
    CMV
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pCI.neo-rPAM1-M314I

    Plasmid
    #145802
    Purpose
    expresses catalytically inactive rat PAM-1 in mammalian cells
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    Expression
    Mammalian
    Mutation
    changed methionine 314 to isoleucine to inactivat…
    Available since
    June 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

    Plasmid
    #89994
    Purpose
    Donor template for generation of NGN3-ntdTomato reporter cell lines
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
    Use
    CRISPR
    Promoter
    none
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pRC/CMV-TYK2K930R-FF-VSV

    Plasmid
    #139348
    Purpose
    Expression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loop
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    Tags
    VSV
    Expression
    Mammalian
    Mutation
    V362F-K930R-Y1054F-Y1055F
    Promoter
    CMV
    Available since
    March 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

    Plasmid
    #111923
    Purpose
    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CeNL(Ca2+)_19μ
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, F92W, E104D, D133E at CaM
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. mCherry-FKBP-SAC1C389S-HLx8

    Plasmid
    #108138
    Purpose
    Recruitable inactive SAC1 with extended linker
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
    Expression
    Mammalian
    Mutation
    SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. mCherry-FKBP-SAC1-HLx2

    Plasmid
    #108133
    Purpose
    Recruitable SAC1 with extended linker
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587) (SACM1L Human)
    Expression
    Mammalian
    Mutation
    SACM1L(1-520):[EAAAR]2:SACM1L(521-587)
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pDual-eGFP(W66)

    Plasmid
    #63217
    Purpose
    Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Insert
    His-T7-eGFP(W66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

    Plasmid
    #58414
    Purpose
    Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells
    Insert
    IFITM3 (Ifitm3 Mouse)
    Tags
    HA internal
    Expression
    Mammalian
    Mutation
    Cysteines 71, 72, and 105 to Alanine; The sequenc…
    Promoter
    CMV IE
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 10621 - 10640 of 16183 results