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Lenti-sgZmat3.55.9/Cre

Plasmid

#167853

Purpose

Expresses Cre recombinase and an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 R72L-T2A-GFP

Plasmid

#163784

Purpose

Expression of R72L CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

R72L

Promoter

MND

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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R701-X56-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D

Plasmid

#159564

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G13D

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X22-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D

Plasmid

#159571

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G13D

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X31-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Plasmid

#159551

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Q61L

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X13-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R

Plasmid

#159545

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Q61R

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X12-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L

Plasmid

#159544

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Q61L

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X27-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C

Plasmid

#159554

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

G12C

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X30-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

Plasmid

#159550

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G13D

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X53-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C

Plasmid

#159561

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12C

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X29-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V

Plasmid

#159556

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

G12V

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Plasmid

#159558

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

Q61L

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA

Plasmid

#113697

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTN-Y117C-FL

Plasmid

#110219

Purpose

Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Tags

His6 and ZB

Expression

Bacterial

Mutation

Y117C, P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti_MS2_P65_HSF1_2A_puro

Plasmid

#118699

Purpose

To express MS2-P65-HSF1

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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HA-MCPIP1

Plasmid

#127459

Purpose

Expressing human MCPIP1 in mammalian cells

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-soCoChR-mCardinal

Plasmid

#107713

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. hSynapsin promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-mCardinal

Use

AAV

Tags

KA2 and mCardinal

Expression

Mammalian

Promoter

hSyn

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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F40 tension sensor

Plasmid

#101252

Purpose

Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-F40-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-IY HA-GBRPL2

Plasmid

#89298

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

HA and IRES-YFP

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-1α

Plasmid

#214341

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1A (IL1A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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