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pLPC-N-Myc-Flag-BirA-hPOT1-DeltaOB

Plasmid

#166410

Purpose

express BirA-hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

POT1 ΔOB (POT1 Human)

Tags

MYC-flag-BirA

Expression

Mammalian

Mutation

ΔOB

Promoter

cmv

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shAspm

Plasmid

#160947

Purpose

Aspm shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Aspm shRNA (Aspm Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_1-274_AG

Plasmid

#148750

Purpose

Mammalian Expression of HsNot4_1-274

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_1-274 (CNOT4 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-SERCA1

Plasmid

#109552

Purpose

Multisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

sarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-Unstructured-EIF3B-465-552

Plasmid

#136029

Purpose

EIF3B (465-552) 3'UTR with the Artificial Unstructured 3'UTR fused upstream inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Delta-Hairpin

Plasmid

#136030

Purpose

EIF3B (465-552) 3'UTR with the main hairpin deleted inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-1st-Hairpin

Plasmid

#136031

Purpose

EIF3B (465-552) 3'UTR with only the main hairpin inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Disrupted-Hairpin

Plasmid

#136032

Purpose

EIF3B (465-552) 3'UTR with the main hairpin mutated inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Restored-Hairpin

Plasmid

#136033

Purpose

EIF3B (465-552) 3'UTR with the main hairpin restored inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-A:U-Hairpin

Plasmid

#136034

Purpose

EIF3B (465-552) 3'UTR with the main hairpin mutated to A and U nucleotides only inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-465-552-Unstructured

Plasmid

#136028

Purpose

EIF3B (465-552) 3'UTR with the Artificial Unstructured 3'UTR fused downstream inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMS22

Plasmid

#129710

Purpose

Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)

Tags

10-Histidine tag cleavable with PreScission Prote…

Expression

Bacterial

Mutation

RssB E134G:E135S:E136G:E137S

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-HA-Tbkbp1 1-280

Plasmid

#125178

Purpose

Retroviral expression of mouse Tbkbp1 (1-280)

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Contains amino acids 1-280

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-HA-Tbkbp1 1-330

Plasmid

#125179

Purpose

Retroviral expression of mouse Tbkbp1 (1-330)

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Contains amino acids 1-330

Promoter

CMV

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pVL43 - pNST3>>

Plasmid

#116009

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pVL42 - pLTP1>>

Plasmid

#116008

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pLTP1:GR-LhG4:tLT1:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37delta2-81

Plasmid

#113502

Purpose

Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 2-81

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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RCH1:GA2ox2-GFP

Plasmid

#108567

Purpose

Arabidopsis transformation, expresses GA2ox2 with RCH1 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

GA2ox2 (GA2OX2 Mustard Weed)

Expression

Plant

Promoter

RCH1

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT1:HDT1-GFP

Plasmid

#108565

Purpose

Arabidopsis transformation, expresses HDT1/GFP fusion with HDT1 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT1 (HDA3 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

HDT1

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT1:GUS

Plasmid

#108441

Purpose

Arabidopsis transformation, expresses HDT1 promoter/GUS fusion to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT1 promoter (HDA3 Mustard Weed)

Tags

GUS

Expression

Plant

Promoter

HDT1

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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