We narrowed to 14,166 results for: flag

1. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

   Plasmid

   #174899

   Purpose

   Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP2135

   Plasmid

   #72248

   Purpose

   Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, and D1135E

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-D2s-L-Venus

   Plasmid

   #19966

   Depositor

   Jonathan Javitch

   Article

   Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

   Insert

   D2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
   

   Tags

   Flag and monomeric Venus

   Expression

   Mammalian

   Available Since

   Jan. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR221-YAP1

   Plasmid

   #79503

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   FLAG

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GPRC5D-Tango

   Plasmid

   #66385

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPRC5D (GPRC5D Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3 merlin 1-595

   Plasmid

   #11623

   Depositor

   Vijaya Ramesh

   Article

   Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

   Insert

   merlin (NF2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pECE M2-SNX17 WT

   Plasmid

   #69811

   Purpose

   Expresses M2-SNX17 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Sorting nexin-17 (SNX17 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   SV40 early promoter

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB412

   Plasmid

   #74806

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBTet-SNAP-CP110

   Plasmid

   #136802

   Purpose

   Mammalian expression of the centrosomal protein Cep110 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-CP110 (CCP110 Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GPR150-Tango

    Plasmid

    #66326

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR150 (GPR150 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. NPS-Tango

    Plasmid

    #66452

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    NPS (NPSR1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pEF1a_FL MUT TET1

    Plasmid

    #124081

    Purpose

    Expression of catalytic inactive form of full-length TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - full length, mutated (TET1 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

    Plasmid

    #186699

    Purpose

    Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBTet-SNAP-Cep131

    Plasmid

    #136826

    Purpose

    Mammalian expression of the centrosomal protein Cep131 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep131 (CEP131 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. NMUR1-Tango

    Plasmid

    #66446

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    NMUR1 (NMUR1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pEBTetBl-CLIP-Cep131

    Plasmid

    #136875

    Purpose

    Mammalian expression of the centrosomal protein Cep131 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep131 (CEP131 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGWB410

    Plasmid

    #74804

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL-CRISPR.SFFV.PAC

    Plasmid

    #57829

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-nLight3.1

    Plasmid

    #111061

    Purpose

    Expresses nLight3.1 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    nLight3.1
    

    Tags

    Flag tag

    Expression

    Mammalian

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GPR173-Tango

    Plasmid

    #66338

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR173 (GPR173 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only