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We narrowed to 14,960 results for: flag

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Showing: 10761 - 10780 of 14960 results

  1. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135014-plasmid-map-sequence-id-267599

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  2. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid
    #135012
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135012-plasmid-map-sequence-id-267596

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  7. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid
    #117070
    Purpose
    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection
    Depositor
    Kim Failor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    SacB
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117070-plasmid-map-sequence-id-231696

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  9. pCEP4-gp120(BaL)-CD4

    Plasmid
    #98957
    Purpose
    Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate
    Depositor
    Erik Procko
    Publication
    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (BaL) gp120
    Tags
    CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    98957-plasmid-map-sequence-id-193406

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  11. ciCas9(F22)_pcDNA5

    Plasmid
    #100552
    Purpose
    Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
    Depositor
    Dustin Maly
    Publication
    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ciCas9(F22)
    Use
    CRISPR; Frt, flp-in
    Tags
    FLAG
    Expression
    Mammalian
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100552-plasmid-map-sequence-id-193861

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  12. SPIN2B (SPIN2BA-c011)

    Plasmid
    #98238
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPIN2BA (SPIN2B Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98238-plasmid-map-sequence-id-193417

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  15. LP455

    Plasmid
    #90198
    Purpose
    pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 649-1426
    Depositor
    Lotte Pedersen
    Publication
    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NPHP4 (NPHP4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    aa residues 649-1426
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90198-plasmid-map-sequence-id-178306

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  16. pAcrF1

    Plasmid
    #89233
    Purpose
    Plasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site
    Depositor
    Blake Wiedenheft
    Publication
    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AcrF1
    Tags
    6xHis-TEV and FLAG
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89233-plasmid-map-sequence-id-177171

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  20. pTC-CMV-Tet-YAP

    Plasmid
    #85579
    Purpose
    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (CMV promoter ) in mouse liver
    Depositor
    Ursula Ehmer
    Publication
    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg
    Inserts
    CreER
    YAP
    Use
    Cre/Lox
    Tags
    Flag (2x)
    Expression
    Mammalian
    Promoter
    TRE
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85579-plasmid-map-sequence-id-169835

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Showing: 10761 - 10780 of 14960 results