We narrowed to 3,063 results for: ER

Showing: 1061 - 1080 of 3063 results

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pFS322-USP44-catDeadMyc

Plasmid

#43904

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Tags

HIS and MYC

Expression

Mammalian

Mutation

C282A, T91A

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pFS321-pDestMyc-USP44catDead

Plasmid

#43902

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Expression

Mammalian

Mutation

C282A

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pFSW Syt1-C2B R398,399Q IRES GFP

Plasmid

#133819

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

R398,399Q

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B K326,327E IRES GFP

Plasmid

#133820

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

K326,327E

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B D363,365N IRES GFP

Plasmid

#133822

Purpose

Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

D363,365N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A D230,232N IRES GFP

Plasmid

#133824

Purpose

Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

D230,232N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2s

Plasmid

#105067

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2s

Tags

6x His tag

Expression

Bacterial

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2.1s

Plasmid

#105068

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2.1s

Tags

6x His tag

Expression

Bacterial

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-deltaTMD SYT7alpha

Plasmid

#179718

Purpose

Lentiviral expression of synaptic vesicle-targeted mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

Enlarge
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD

Plasmid

#179722

Purpose

Lentiviral expression of lysosome-targeted mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

Enlarge
pF(UG) U6-SYT7 sgRNA 777 HaloTag

Plasmid

#179724

Purpose

U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

sgRNA and HaloTag

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pF(UG) CamKII sf iGluSnFR S72A

Plasmid

#179726

Purpose

Lentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

iGluSnFR

Use

Lentiviral

Mutation

S72A

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D303G F234C

Plasmid

#170647

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D303G, C277A, F234C

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D303G V304C

Plasmid

#170648

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D303G, C277A, V304C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) D365E F234C

Plasmid

#170650

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D365E, C277A, F234C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) D365E V304C

Plasmid

#170651

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D365E, C277A, V304C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) I367T F234C

Plasmid

#170653

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

I367T, C277A, F234C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) I367T V304C

Plasmid

#170654

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

I367T, C277A, V304C

Availability

Academic Institutions and Nonprofits only

Enlarge
pET SUMO-FL-syt1

Plasmid

#213628

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt7

Plasmid

#213630

Purpose

Bacterial expression of SUMO-tagged mouse FL-syt7

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt7 (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge