We narrowed to 3,041 results for: ER;

1. pFSW Syt1-C2B D363,365N IRES GFP

   Plasmid

   #133822

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   D363,365N

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFSW Syt1-C2A D230,232N IRES GFP

   Plasmid

   #133824

   Purpose

   Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   D230,232N

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Syt1 C2AB (96-421) D303G F234C

   Plasmid

   #170647

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   D303G, C277A, F234C

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX Syt1 C2AB (96-421) D303G V304C

   Plasmid

   #170648

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   D303G, C277A, V304C

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX Syt1 C2AB (96-421) D365E F234C

   Plasmid

   #170650

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   D365E, C277A, F234C

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX Syt1 C2AB (96-421) D365E V304C

   Plasmid

   #170651

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   D365E, C277A, V304C

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX Syt1 C2AB (96-421) I367T F234C

   Plasmid

   #170653

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   I367T, C277A, F234C

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX Syt1 C2AB (96-421) I367T V304C

   Plasmid

   #170654

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   I367T, C277A, V304C

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-VSVG

   Plasmid

   #11912

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

   Insert

   VSVG
   

   Expression

   Mammalian

   Mutation

   F64L, S65T, A206K relative the wild type GFP sequ…

   Available Since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMAL

    Plasmid

    #161785

    Purpose

    Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Bidirectional minhCMV and SFFV

    Available Since

    Dec. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB_tre_dCas9_KRAB

    Plasmid

    #126030

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with KRAB
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag

    Plasmid

    #179721

    Purpose

    Lentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N2-COL1A1

    Plasmid

    #66602

    Purpose

    Expresses procollagen 1 alpha 1 as a GFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Expression

    Mammalian

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Rtn4a-mNeonGreen

    Plasmid

    #169032

    Purpose

    Express human Rtn4a

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rtn4a (RTN4 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2 Lnp-mCherry

    Plasmid

    #86687

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    LNPK (LNPK Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMTS-HA-GFP11

    Plasmid

    #177725

    Purpose

    Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    MTS-HA-GFP11
    

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Neo-Bam p53 R175H

    Plasmid

    #16436

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R175H (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    CMV

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30_Halotag_K73T_v2

    Plasmid

    #183692

    Purpose

    Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo(K73T)
    

    Expression

    Bacterial

    Mutation

    K73T in Halotag

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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