We narrowed to 5,441 results for: SPI

1. pCS2-phiC31o (JDW 453)

   Plasmid

   #229842

   Purpose

   A CMV driven Phi31 recombinase.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   phiC31 codon optimized, PhiC31o
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-V5-mTagBFP2 (JDW 871)

   Plasmid

   #224531

   Purpose

   A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   V5-mTagBFP2
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. LCB1_v1.3 (MTK 3a) (pZYW044)

   Plasmid

   #194227

   Purpose

   Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   LCB1_v1.3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LCB3_v1.2 (MTK 3a) (pZYW045)

   Plasmid

   #194228

   Purpose

   Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   LCB3_v1.2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4/TO-Cdc45HA

   Plasmid

   #211427

   Purpose

   tet-inducible mammalian expression of human Cdc45 C-terminally fused to HA-Tag

   Depositor

   Helmut Pospiech

   Article

   Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

   Insert

   Cdc45 (CDC45 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.11_Nued2_E1_dAP1-2

   Plasmid

   #203837

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dAP1-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   KO of both AP1 sites

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.11_Nued2_E1_dAP1

   Plasmid

   #203835

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dAP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   KO of AP1 upstream

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL4.11_Nued2_E1_dAP2

   Plasmid

   #203836

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 site

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dAP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   KO of AP1 downstream

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCZGY921

   Plasmid

   #135092

   Purpose

   Transcriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegans

   Depositor

   Yishi Jin

   Article

   Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

   Insert

   Pacr-2
   

   Use

   Tags

   GFP and Synthetic Intron

   Expression

   Worm

   Mutation

   Promoter

   Pacr-2

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGEN-WPRE-bGH-DEST (JDW 1309)

    Plasmid

    #229815

    Purpose

    A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-3x-FLAG-Ubiquitin (JDW 1098)

    Plasmid

    #224513

    Purpose

    A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    3x-FLAG-Ubiquitin
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Ef1a-fDIO-mRuby3-2A-Cre

    Plasmid

    #203844

    Purpose

    Flp-dependent Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-BLU-VIPR

    Plasmid

    #220497

    Purpose

    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBLU-VIPR

    Plasmid

    #220498

    Purpose

    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

    Plasmid

    #203842

    Purpose

    Flp-dependent Cre and EGFP expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    EGFP-2A

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-f:PGC1

    Plasmid

    #1026

    Depositor

    Bruce Spiegelman

    Article

    Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

    Insert

    PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQCXIP-cathepsinD-YMH

    Plasmid

    #182644

    Purpose

    Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    cathepsinD
    

    Use

    Retroviral

    Tags

    His6-tag, TS site from proCCK, and myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

    Plasmid

    #203838

    Purpose

    Flp-dependent Cre and mScarlet-KASH expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    mScarlet-KASH-2A

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pME-mScarlet3-Giantin (JDW 1356)

    Plasmid

    #224502

    Purpose

    Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    mScarlet3-Giantin
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV_NFL(WT)-FLAG

    Plasmid

    #182654

    Purpose

    Expresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    wild type mouse neurofilament light chain (Nefl Mouse)
    

    Use

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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