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pET29a-hNABP2-N

Plasmid

#128308

Purpose

Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 N terminal fragment (OB fold) (NABP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (1–138 aa)

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PL-452 N-CyPet

Plasmid

#19171

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

Insert

CyPet

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET41a iGlul

Plasmid

#119832

Purpose

Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGlul

Use

Tags

GST

Expression

Bacterial

Mutation

T92A

Promoter

T7

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2NhNABP1C

Plasmid

#128313

Purpose

Express chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP2N+hNABP1C (NABP2, NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP2N+hNABP1C, Y85C (please se…

Promoter

T7

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET14b-mEKLF

Plasmid

#67834

Purpose

Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution

Depositor

James Bieker

Article

Armstrong et al Cell. 1998 Oct 2;95(1):93-104.

Insert

EKLF (Klf1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

T7

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-λN-βLac

Plasmid

#162039

Purpose

Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)

Depositor

Karen Polizzi

Article

Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199

Insert

λN domain of the λ phage and a β-lactamase fusion protein

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-clbB

Plasmid

#114155

Purpose

Expresses full-length ClbB

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbB

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.d

Plasmid

#176213

Purpose

Bacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.d

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-mxLOX1

Plasmid

#104975

Purpose

Biosynthesis of oxylipins by microbial enzymes

Depositor

Deokkun Oh

Insert

MXAN_1745 (MXAN_1745 Myxococcus xanthus)

Use

Tags

6 histidine tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-mxLOX2

Plasmid

#104976

Purpose

Biosynthesis of oxylipins by microbial enzymes

Depositor

Deokkun Oh

Insert

MXAN_1744 (MXAN_1744 Myxococcus xanthus)

Use

Tags

6 histidine tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-MtrR

Plasmid

#51188

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

MtrR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET21-MRPS2

Plasmid

#31342

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein S2 mature form (MRPS2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Encodes the predicted mature form for incorporati…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
6107 pETH6KT218

Plasmid

#1241

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (K218T) (HSP104 Budding Yeast)

Use

Tags

His tag

Expression

Bacterial

Mutation

K218T

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.t

Plasmid

#176214

Purpose

Bacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.t

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-pchA WT

Plasmid

#87102

Purpose

expresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Arch Biochem Biophys. 2013 Oct 1;538(1):

Insert

pchA WT

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-JadR2

Plasmid

#51178

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

JadR2

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b-At 14-3-3 omicron

Plasmid

#44926

Depositor

Robert Ferl

Insert

14-3-3 omicron (GRF11 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 phi

Plasmid

#44939

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 phi (GF14 PHI Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (P to A)

Plasmid

#62069

Purpose

Expression of human p53 (1-73) (all P to A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; all Prolines changed …

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET23a-MmUBC

Plasmid

#69555

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBC (Ubc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only