We narrowed to 9,995 results for: pET

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pET32a-Spike-S-4P

Plasmid

#165094

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Publication

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)

Expression

Bacterial

Available since

March 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET30a-FluB-NP

Plasmid

#165093

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Publication

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza B virus(B/Georgia/05/2019) nucleocapsid protein

Expression

Bacterial

Available since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET11a-STEPtag

Plasmid

#159373

Purpose

Expresses STEPtag in E. coli

Depositor

Roberto Chica

Publication

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEThT-SAHS2

Plasmid

#90026

Purpose

To express His-tagged SAHS2 in bacteria

Depositor

Takekazu Kunieda

Publication

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

SAHS2

Tags

His

Expression

Bacterial

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-MRPP1_native+

Plasmid

#67865

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form)

Depositor

Walter Rossmanith

Publication

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP1 (TRMT10C Human)

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET21c-IF3mt

Plasmid

#31078

Depositor

Linda Spremulli

Publication

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Ami…

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET28(a)-hFX-HA

Plasmid

#14991

Depositor

Michael Ristow

Publication

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)

Plasmid

#48322

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Thioredoxin

Expression

Bacterial

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-CohIII-CBM-HIS-LPETGG

Plasmid

#91697

Purpose

CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

Depositor

Hermann Gaub

Publication

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

CohIII-CBM-HIS-LPETGG

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b/EYFP-GgVcl 884-1066-ECFP FRET

Plasmid

#46179

Depositor

Susan Craig

Insert

Vcl 884-1066 (VCL Chicken)

Tags

ECFP, EYFP, and His

Expression

Bacterial

Mutation

aa 884-1066 (Vt, tail domain)

Promoter

T7

Available since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Publication

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ppih.001.177.W133A

Plasmid

#137655

Purpose

Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Publication

Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

Insert

ppih (PPIH Human)

Tags

GST-(His)6-thrombin

Expression

Bacterial

Mutation

001-177-W133A

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBz106_CsoS2_MR

Plasmid

#140843

Purpose

His-tagged CsoS2 Middle Region (MR), pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 Middle Region (MR)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

only amino acids 257-603

Promoter

T7

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMW41: pET-His-MBP-SUMO-R2Bm-SII

Plasmid

#209290

Purpose

Expresses the R2Bm ORF in E. coli

Depositor

Feng Zhang

Publication

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2

Tags

His, MBP, StrepII, and bdSUMO

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3)P Probe

Plasmid

#211508

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

FYVE-Hrs x 2 (PIKFYVE Human)

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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6xHis-SNAP-PI(3,5)P2 Probe

Plasmid

#211512

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SnxA-2xPX

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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PVS10

Plasmid

#104398

Purpose

polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Publication

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Beta-Lactamase

Plasmid

#62729

Purpose

Expresses Beta-Lactamase protein in bacterial cells

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Beta-Lactamase

Tags

His tag

Expression

Bacterial

Mutation

Deletion of periplasmic signal domain

Promoter

T7

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

Plasmid

#198751

Purpose

Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Publication

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - LargeBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+) NRP1 b1 (273-427)

Plasmid

#162734

Purpose

Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Publication

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1 domain residues 273-427

Expression

Bacterial

Mutation

Codon optimised

Available since

April 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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