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pMBS-845

Plasmid

#218000

Purpose

Strep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Strep-tag II

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-846

Plasmid

#218001

Purpose

3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

3xFLAG-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-847

Plasmid

#218002

Purpose

Myc-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Myc-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-848

Plasmid

#218003

Purpose

GST-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

GST-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-849

Plasmid

#218004

Purpose

8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-850

Plasmid

#218005

Purpose

Receptor binding domain of SARS-CoV-2

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

RBD (S SARS-CoV-2)

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-857

Plasmid

#218007

Purpose

Receptor binding domain of SARS-CoV-2

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

RBD (S SARS-CoV-2)

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_005

Plasmid

#180555

Purpose

Entry vector containing Terminator sequence (Bba_B0015)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator sequence (Bba_B0015)

Expression

Bacterial

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_047

Plasmid

#180551

Purpose

Entry vector containing sfGFP in E1.3a format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP in E1.3a format

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_110

Plasmid

#180554

Purpose

Entry vector containing 7xhis tag in E1.3b format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

7xhis tag in E1.3b format

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_007

Plasmid

#180563

Purpose

Entry vector containing Terminator sequence (Bba_B0015)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator sequence (Bba_B0015)

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_044

Plasmid

#180532

Purpose

Entry vector containing J23117 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23117 anderson promoter

Expression

Bacterial

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_043

Plasmid

#180531

Purpose

Entry vector containing J23101 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23101 anderson promoter

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_031

Plasmid

#180556

Purpose

Entry vector containing Terminator - BBa_B0010 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator - BBa_B0010 terminator

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_045

Plasmid

#180543

Purpose

Entry vector containing RBS 30,000 a.u. designed strength

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sRBS 30,000 a.u. designed strength

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_252

Plasmid

#180553

Purpose

Entry vector containing mScarlet in E1.3b format

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

mScarlet in E1.3b format

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_046

Plasmid

#180544

Purpose

Entry vector containing RBS 250 a.u. designed strength

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sRBS 250 a.u. designed strength

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_042

Plasmid

#180530

Purpose

Entry vector containing J23100 anderson promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23100 anderson promoter

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBlastR-MCS-LexA-VP16-MiniWhite

Plasmid

#165900

Purpose

Blasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge