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3x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64391

Purpose

encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Expression

Yeast

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
in-frame ampicillin PKAKTR backbone

Plasmid

#177197

Purpose

PKAKTR mutagenesis template with in-frame ampicillin site

Depositor

Markus Covert

Publication

Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGRNA4e

Plasmid

#169784

Purpose

Insert one gRNA

Depositor

Sang-Gyu Kim

Publication

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Unspecified

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ134-STU-As

Plasmid

#138103

Purpose

Golden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ134-STU-Fn

Plasmid

#138104

Purpose

Golden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-As

Plasmid

#138094

Purpose

Golden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-Fn

Plasmid

#138095

Purpose

Golden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ132-STU-Fn

Plasmid

#138098

Purpose

Golden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133-STU-Fn

Plasmid

#138101

Purpose

Golden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pJKW1659

Plasmid

#119180

Purpose

E.coli expression plasmid for Pm4CL1 and PmCHS

Depositor

Jing-Ke Weng

Publication

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Inserts

Pm4CL1
PmCHS

Expression

Bacterial

Promoter

pGAP

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pYTK049

Plasmid

#65156

Purpose

Encodes I-SceI recognition site as a Type 234 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

I-SceI recognition site

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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LI dy Esp3I-ccdB C-D

Plasmid

#54460

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

ccdB C-D

Use

Cloning vector

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
LI dy Esp3I-ccdB A-B

Plasmid

#54459

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

ccdB A-B

Use

Cloning vector

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pISH-H2-M1

Plasmid

#15867

Depositor

Peter Mombaerts

Publication

Ishii et al Curr Biol. 2003 Mar 4. 13(5):394-400.

Insert

H2-M1 (H2-M1 Mouse)

Available since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only
pUC57KAN-10XALFA

Plasmid

#233106

Purpose

10X ALFA tag multimer

Depositor

Steve Stowers

Insert

ALFA tag

Use

Unspecified

Mutation

None

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK191

Plasmid

#226658

Purpose

Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, GentR, contains HIP1 site

Depositor

David Nielsen

Publication

Kamoku et al Synthetic Biology and Engineering 2024,

Type

Empty backbone

Use

Unspecified

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMYT013

Plasmid

#180666

Purpose

Encodes pTet5 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Publication

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pTet5

Use

Synthetic Biology

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT018

Plasmid

#180671

Purpose

Encodes rtTA as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Publication

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

rtTA

Use

Synthetic Biology

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT015

Plasmid

#180668

Purpose

Encodes pTet7 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Publication

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pTet7

Use

Synthetic Biology

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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NTERY01

Plasmid

#184119

Purpose

Nanopore-addressable protein reporter for expression in bacterial cells.

Depositor

Jeff Nivala

Publication

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

NTERY01

Expression

Bacterial

Promoter

T7

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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