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We narrowed to 60,318 results for: cloning

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Showing: 10881 - 10900 of 60318 results

  1. pKSB-631 U6-sgRNA3/4 AACA GCTT

    Plasmid
    #173675
    Purpose
    To clone and express a gRNA in Anopheles
    Depositor
    Eric Marois
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Insect
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173675-plasmid-map-sequence-id-342964

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  2. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166107-plasmid-map-sequence-id-330320

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  3. pEF 3-CFP-2

    Plasmid
    #20105
    Depositor
    Antonio Richart
    Insert
    CFP (Cfp )
    Use
    Synthetic Biology; Plant gateway vector
    Available Since
    Feb. 12, 2009
    Availability
    Academic Institutions and Nonprofits only
    20105-plasmid-map-sequence-id-9475

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  4. mU6-sgLkb1_1st-hU6-sgNT

    Plasmid
    #177220
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_1st/sgNT1
    Use
    Lentiviral
    Promoter
    mU6/hU6
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177220-plasmid-map-sequence-id-350037

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  5. pIC81

    Plasmid
    #63065
    Purpose
    Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    PMFI, IMAGE clone 6148250 (PMF1 Human)
    Tags
    EYFP and TEV-S
    Expression
    Mammalian
    Available Since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63065-plasmid-map-sequence-id-104380

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  6. NARS_pNIC-Bio3

    Plasmid
    #153054
    Purpose
    Expression construct for producing Avi tagged Asparaginyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    NARS (NARS Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153054-plasmid-map-sequence-id-304473

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  7. IDG_TTYH2_OE_1

    Plasmid
    #161676
    Purpose
    Overexpresses TTYH2
    Depositor
    Mike McManus
    Insert
    TTYH2 (TTYH2 Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    161676-plasmid-map-sequence-id-334544

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  8. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid
    #172298
    Purpose
    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes
    Depositor
    Patrik Verstreken
    Insert
    CRE-T2A-PuroR
    Use
    Cre/Lox
    Promoter
    T7 promoter A-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172298-plasmid-map-sequence-id-340323

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  9. MPSTA

    Plasmid
    #42482
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MPSTA
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE

    Plasmid
    #186047
    Purpose
    AAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    LifeAct-jGCaMP8f
    Use
    AAV
    Tags
    LifeAct-6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186047-plasmid-map-sequence-id-370161

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  11. AAV-CAG-FLEX-LifeAct-jGCaMP8m-WPRE

    Plasmid
    #186048
    Purpose
    AAV-mediated expression of LifeAct-jGCaMP8m under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    LifeAct-jGCaMP8m
    Use
    AAV
    Tags
    LifeAct-6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    186048-plasmid-map-sequence-id-370148

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  12. pDEST26-hTLR3-Nluc

    Plasmid
    #197638
    Purpose
    Human CD283 protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR3 (TLR3 Human)
    Tags
    NanoLuciferase
    Expression
    Mammalian
    Available Since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197638-plasmid-map-sequence-id-392617

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  13. pAW8-aid-3HA-Turg1:kanMX6

    Plasmid
    #169355
    Purpose
    C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    3XHA
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169355-plasmid-map-sequence-id-333206

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  14. pSiM3

    Plasmid
    #199382
    Purpose
    Encodes dual cistronic membrane localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP7s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199382-plasmid-map-sequence-id-391043

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  15. g7s pDONR

    Plasmid
    #117456
    Purpose
    Gateway Vector for Cyth4
    Depositor
    Richard Kahn
    Insert
    Cyth4 (CYTH4 Human)
    Use
    Gateway shuttling vector
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117456-plasmid-map-sequence-id-226839

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  16. C35m

    Plasmid
    #120969
    Purpose
    MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).
    Depositor
    Richard Murray
    Insert
    RhlR
    Use
    Synthetic Biology
    Mutation
    BsaI and BbsI sites removed; amino acid sequence …
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120969-plasmid-map-sequence-id-238056

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  17. pAS1-his-24::tagRFP

    Plasmid
    #124329
    Purpose
    Expressing Nuclear Localized tagRFP
    Depositor
    Andrew Leifer
    Insert
    tagRFP (his-24 Nematode)
    Expression
    Worm
    Available Since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124329-plasmid-map-sequence-id-241910

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  18. pAW8-aid-V5-Turg1:kanMX6

    Plasmid
    #169357
    Purpose
    C-terminal aid-V5 degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    1XV5
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169357-plasmid-map-sequence-id-333200

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  20. pINER2R

    Plasmid
    #185882
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185882-plasmid-map-sequence-id-370829

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Showing: 10881 - 10900 of 60318 results