Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 14,960 results for: flag

Clear keywords
Showing: 10881 - 10900 of 14960 results

  1. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

    Enlarge

  2. HA Display H3_A/Hong Kong/1/1968

    Plasmid
    #206506
    Purpose
    Expresses H3_Hong Kong/1/1968 in the surface of mammalian cells
    Depositor
    IIya Finkelstein
    Insert
    H3_A/Hong Kong/1/1968
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    deleted amino acids 1651 to 1735
    Promoter
    CMV
    Available since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    206506-plasmid-map-sequence-id-408102

    Enlarge

  4. NES-Halo

    Plasmid
    #200201
    Purpose
    Expression of 3X HIV NES
    Depositor
    Sheila Teves
    Publication
    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3X HIV Rev NES-Halo
    Tags
    Halo
    Expression
    Mammalian
    Mutation
    None
    Promoter
    EF_1alpha
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200201-plasmid-map-sequence-id-393124

    Enlarge

  7. HsSPHK2

    Plasmid
    #118092
    Purpose
    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Kevin Lynch
    Publication
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    GAL1, 10
    Available since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    118092-plasmid-map-sequence-id-234125

    Enlarge

  8. pSN840

    Plasmid
    #184832
    Purpose
    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells
    Depositor
    Kyoko Chiba
    Publication
    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g
    Inserts
    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    Use
    Cre/Lox
    Tags
    His-FLAG and mScarlet-2xStrepII
    Expression
    Insect
    Mutation
    ∆exon27 form of human KIF5A
    Promoter
    p10 and polyhedrin
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    184832-plasmid-map-sequence-id-367218

    Enlarge

  9. pMX-53BP1(1-1710) 3LA

    Plasmid
    #185698
    Purpose
    Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.
    Depositor
    Daniel Durocher
    Publication
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    L175A, L517A, and L693A
    Promoter
    SV40
    Available since
    July 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185698-plasmid-map-sequence-id-365946

    Enlarge

  10. pMX-53BP1(1-1710) 3LA7A

    Plasmid
    #185707
    Purpose
    Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.
    Depositor
    Daniel Durocher
    Publication
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA7A (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    T302A, S452A, S523A, S543A, S625A, S784A, and S89…
    Promoter
    SV40
    Available since
    July 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185707-plasmid-map-sequence-id-365947

    Enlarge

  15. NLS-4LNDD-pcDNA3.1(+)

    Plasmid
    #172313
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells
    Depositor
    Joshua Rosenthal
    Publication
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172313-plasmid-map-sequence-id-339585

    Enlarge

  18. pENTR1A mKate2-hu_ncOGT D554N

    Plasmid
    #154283
    Purpose
    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)
    Depositor
    Suzanne Walker
    Publication
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Gateway Cloning
    Tags
    2x FLAG and mKate2
    Mutation
    D554N mutation (Ser/Thr Glycosylation Impaired)
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154283-plasmid-map-sequence-id-304858

    Enlarge

  19. pTXB1-TP4

    Plasmid
    #160087
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4
    Depositor
    Pier Pandolfi
    Publication
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160087-plasmid-map-sequence-id-313735

    Enlarge

Showing: 10881 - 10900 of 14960 results