We narrowed to 88,516 results for: MAL

1. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

   Plasmid

   #173029

   Purpose

   For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   pspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, BFP, HIV NES, and T2A

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-sgRNA blast

   Plasmid

   #104993

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PE6b

   Plasmid

   #207852

   Purpose

   Mammalian expression of PE6b prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6b
   

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PEmax-P2A-GFP

   Plasmid

   #180020

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-EGFP
   

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-KDEL_CD9-SBP-mCherry

   Plasmid

   #222329

   Purpose

   Synchronize the trafficking of CD9 from the ER.

   Depositor

   Franck Perez

   Article

   Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

   Insert

   Streptavidin-KDEL and CD9 fused to SBP-mCherry (CD9 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Str-KDEL_CD9-SBP-EGFP

   Plasmid

   #222328

   Purpose

   Synchronize the trafficking of CD9 from the ER.

   Depositor

   Franck Perez

   Article

   Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

   Insert

   Streptavidin-KDEL and CD9 fused to SBP-EGFP (CD9 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-sgRNA puro

   Plasmid

   #104990

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBbleo-EREG-ScNeo

   Plasmid

   #209905

   Purpose

   To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   EREG-ScNeo (EREG Human)
   

   Tags

   GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-Cas9-2A-BFP

   Plasmid

   #164662

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Inserts

   Cas9
   BFP
   

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   MSSV_LTR

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-sgRNA hygro

    Plasmid

    #104991

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid

    #140982

    Purpose

    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaQ-RLuc8 (GNAQ Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti PGK FKBP12(F36V)-OGT (Neo)

    Plasmid

    #154294

    Purpose

    Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139093

    Purpose

    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dxCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ezh2[FL]-dCas9

    Plasmid

    #100086

    Purpose

    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    14056 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[FL]-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. DNMT3A-dCas9

    Plasmid

    #100090

    Purpose

    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-DNMT3A-dCas9-NLS

    Expression

    Mammalian

    Mutation

    includes aa 602-912

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-dCas13-M3M14nes

    Plasmid

    #155367

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXS-mitoGshF-blasticidin

    Plasmid

    #210314

    Purpose

    Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells

    Depositor

    Kivanc Birsoy

    Article

    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

    Insert

    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    

    Use

    Retroviral

    Tags

    FLAG peptide, Homo sapiens ACO2 mitochondrial tar…

    Mutation

    Codon optimized for expression in human cells

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCMV_HyPer7RgDAAO

    Plasmid

    #217653

    Purpose

    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    HyPer7 D amino acid oxidase
    

    Use

    Lentiviral

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    Fused DAAO to the C-terminus of HyPer7 using a Gl…

    Promoter

    CMV

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

    Plasmid

    #177120

    Purpose

    dCasRx mediated RNA tethering of FTO

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    dCasRx-FTO
    

    Use

    Lentiviral

    Tags

    HA and SV40 NLS

    Mutation

    dCasRX contains R239A/H244A/R858A/H863A mutations…

    Promoter

    Ef1a

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid

    #188899

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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