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pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

Plasmid

#189633

Purpose

Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLeo-A-GEMS(JAK-STAT)

Plasmid

#209109

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-A-a3-GEMS(JAK-STAT)

Plasmid

#209111

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a3-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a3-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-B-a2-GEMS(JAK-STAT)

Plasmid

#209112

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor B-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

B-a2-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-G-a2-GEMS(JAK-STAT)

Plasmid

#209113

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor G-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

G-a2-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l2-A'-IRES-EmGFP

Plasmid

#209125

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-A'-IRES-EmGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-MSI1-ZF

Plasmid

#200912

Purpose

Express MSI1-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Article

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

MSI1 (MSI1 Mustard Weed)

Expression

Plant

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

Plasmid

#189632

Purpose

Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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rUBR5_2383-2442_pGEX6P1

Plasmid

#209791

Purpose

Bacterial expression of UBR5 Mlle domain

Depositor

Kalle Gehring

Article

Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

Insert

Mlle domain of UBR5 (Ubr5 Rat)

Tags

GST

Expression

Bacterial

Mutation

aa 2383-2442 only

Promoter

tac

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)

Plasmid

#209776

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

Nluc, sgSTK4i, mRuby2 (STK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-KRAB_sgSTK4i_#1 (PuroR)

Plasmid

#209773

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-KRAB, sgSTK4i (STK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-KRAB_sgSTK4i_#2 (PuroR)

Plasmid

#209774

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-KRAB, sgSTK4i (STK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D197, 199N IRES GFP

Plasmid

#195701

Purpose

Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D197, 199N mutations

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D330, 332N IRES GFP

Plasmid

#195702

Purpose

Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D330, 332N mutations

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_TAOK2-D151A

Plasmid

#172980

Purpose

doxycycline-inducible expression of kinase-dead TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

kinase-dead TAOK2 (TAOK2-D151A) (TAOK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

D151A

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#1

Plasmid

#172983

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-IGFBP2-Clover

Plasmid

#191007

Purpose

Gateway entry vector with IGFBP2 tagged with Clover

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Gateway entry vector

Tags

Clover

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mCsnk2b - 2

Plasmid

#198506

Purpose

lentiviral stable expression of mCsnk2b gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mCsnk2b (Csnk2b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgINTS12 guide 2

Plasmid

#193607

Purpose

INTS12 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgINTS12 guide 2 (INTS12 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgINTS12 guide 1

Plasmid

#193606

Purpose

INTS12 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgINTS12 guide 1 (INTS12 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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