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pET-20b-T7-Ntx-K49

Plasmid

#58715

Purpose

Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Tags

T7 peptide tag

Expression

Bacterial

Mutation

Changed Aspartic Acid 49 to Lysine

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pSC-mTDG-K341R-IntE2

Plasmid

#52269

Purpose

Bacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

TDG (Tdg Mouse)

Tags

GyrA-intein-Ubc9-GST

Expression

Bacterial

Mutation

K341R, SUMO acceptor site mutation

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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Hoxd13-3'UTR

Plasmid

#31525

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

Hoxd13-3'UTR (Hoxd13 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
SNX9-PX (246-376)

Plasmid

#119090

Purpose

Bacterial expression of human phox homology (PX) domain, SNX9-PX (246-376)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX9-PX (246-376) (SNX9 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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PI3KC2gamma-PX (1200-1310)

Plasmid

#119121

Purpose

Bacterial expression of human phox homology (PX) domain, PI3KC2gamma-PX (1200-1310)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

PI3KC2gamma-PX (1200-1310) (PIK3C2G Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta 6X mutant

Plasmid

#128816

Purpose

To make virus expressing Doc2beta 6X mutant

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Mutation

D163A, D218A, D220N, D303A, D357A, D359A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

Plasmid

#141395

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

eGFP

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-VPR

Plasmid

#63800

Purpose

SP-dCas9-VPR with doxycycline-inducible expression

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCbs FlagOmomyc

Plasmid

#113168

Purpose

Expression of FLAG tagged Omomyc in mammalian cells

Depositor

Sergio Nasi

Article

Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

Insert

Omomyc (MYC Human)

Tags

FLAG

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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TRPML1-G-GECO1.2-ERES

Plasmid

#207144

Purpose

Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Mcoln1 (MCOLN1 Human)

Tags

ER-Export Signal (ERES) and G-GECO1.2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 IP3R3 WT

Plasmid

#236453

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pKC117_pLenti_TetOff_MVP_IRES_PABPC1_INT

Plasmid

#242113

Purpose

Tet- or Dox-repressible expression of MVP and PABPC1 fused to INT (human PARP4 fragment)

Depositor

Fei Chen

Article

Chao et al Science. 2026 Jan 15:eadz9353. doi: 10.1

Insert

MVP and PAB4L-PARP4 fragment (MVP, PABPC1 Synthetic)

Use

Lentiviral

Mutation

PABPC1 aa 1-370, PARP4 aa 1562-1724

Promoter

TRE3G

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

Plasmid

#223724

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FIP200-CTR (RB1CC1 Human)

Tags

6xHis-TEV-EGFP

Expression

Bacterial

Mutation

aa 1429-1591 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST RBM14-V5

Plasmid

#69823

Purpose

Expresses RBM14-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

RNA-binding protein 14 (RBM14 Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A

Plasmid

#145374

Purpose

expresses human Nav1.5 (WT) in mammalian cells

Depositor

Hugues Abriel, Robert Kass

Article

Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pABE8e-NG-403Q-Nterm-AAV

Plasmid

#194651

Purpose

N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

Depositor

David Liu

Article

Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

Insert

ABE8e-NG N-term

Use

AAV

Tags

BPNLS and NpuN spilt intein

Promoter

TNNT2

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pABE8e-NG-403Q-Cterm-AAV

Plasmid

#194652

Purpose

C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

Depositor

David Liu

Article

Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

Insert

ABE8e-NG C-term

Use

AAV

Tags

BPNLS and NpuC split intein

Promoter

TNNT2

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D hM3D mCherry SV40

Plasmid

#92284

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

hM3D (CHRM3 Human)

Use

AAV

Tags

mCherry

Promoter

GfaABC1D

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D hM4D mCherry SV40

Plasmid

#92286

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

hM4D (CHRM4 Human)

Use

AAV

Tags

mCherry

Promoter

GfaABC1D

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR

Plasmid

#28235

Purpose

Mammalian expression of Androgen receptor fused to EGFP

Depositor

Michael Mancini

Article

Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

Insert

Androgen receptor (AR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only

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