We narrowed to 852 results for: VILL

1. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

   Plasmid

   #188541

   Purpose

   Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2/GD-IRES-GFP-CTNNB1

   Plasmid

   #192868

   Purpose

   Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

   Depositor

   David Largaespada

   Article

   Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

   Inserts

   luc+
   EGFP
   CTNNB1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-DNAJC5 (L115R)

   Plasmid

   #205728

   Purpose

   Mammalian expression of DNAJC5 (L115R)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (L115R) (DNAJC5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. hPlekhm1-dsRedM

   Plasmid

   #73592

   Purpose

   Express human Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   dsRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

5. CIAR_pcDNA5/FRT/TO

   Plasmid

   #86502

   Purpose

   Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CIAR (SOS1 Human)
   

   Use

   Frt

   Tags

   Expression

   Mammalian

   Mutation

   T968L in SOScat

   Promoter

   CMV (tet operator)

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiV_Neo_SIK3_CR_T221A

   Plasmid

   #108107

   Purpose

   Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   change guanine 501 to cytosine (silent mutation),…

   Promoter

   EFS promoter

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T-3-TRC8 513-664

   Plasmid

   #185352

   Purpose

   RING domain-containing GST fusion protein for ubiquitination assays.

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   TRC8 (RNF139 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 513-664 only

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET3a-UBE2J1 1-283

   Plasmid

   #185348

   Purpose

   In vitro transcription and translation in bacterial system

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   UBE2J1 (UBE2J1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cytoplasmic domain only, aa 1-283

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL-p21UTRm1

   Plasmid

   #20878

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR site 1 mutation (Cdkn1a Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Predicted miRNA binding site 1 (Position ~420) GC…

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pLV-CMV-EGFP-PolB-Hygro

    Plasmid

    #176056

    Purpose

    EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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