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pIN3_enpp-1_mScarlet

Plasmid

#182934

Purpose

Expresses enpp-1 (C27A7.1) under its native promoter (male reproductive tract and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

genomic enpp-1 fused to mScarlet

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Promoter

enpp-1

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pIN2_mcm-3_mScarlet

Plasmid

#182933

Purpose

Expresses mcm-3 under its native promoter (embryonic nuclei and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

genomic mcm-3 fused to mScarlet

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Promoter

mcm-3

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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KG#797

Plasmid

#110916

Purpose

Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

Inserts

unc-129 promoter
STRN-1 (Sentryn)-GFP
unc-54 3' control region with 1 intron just upstream and 1 intron in control region

Tags

GFP (contains 3 artificial introns)

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1827

Plasmid

#154323

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I

Use

CRISPR

Expression

Worm

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1841

Plasmid

#154338

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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AP54-5

Plasmid

#65599

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs12

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
AP56-6

Plasmid

#65593

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP56-5

Plasmid

#65591

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP56-8

Plasmid

#65596

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
AP56-7

Plasmid

#65594

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only