Addgene

We narrowed to 4,334 results for: 187

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pDest17_Gcn4 P+

Plasmid

#231872

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 P+

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 P+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S122P, T131F, A141P, E143D

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3 W

Plasmid

#231876

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3 W

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 R3 W

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, N126W, D133R, K143R

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R2

Plasmid

#231870

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R2

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 R2

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

K118R, K140R

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::FRT-RC,attB}

Plasmid

#182187

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046678

Plasmid

#149187

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF10 protein (ORF10 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-GFP-CENPBpA-fixed-length-clone-9

Plasmid

#120187

Purpose

Randomly picked clone 9 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSGE_892_Nhe2_EcR-to-HLH4C_RB

Plasmid

#71187

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

892_Nhe2_EcR-to-HLH4C_RB

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pmU6-gRNA

Plasmid

#53187

Purpose

Expresses the S. pyogenes sgRNA from the mouse U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAX1

Plasmid

#117397

Purpose

allelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replication

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 hSmad1-MM

Plasmid

#22990

Depositor

Edward De Robertis

Article

Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

Insert

Smad1 (SMAD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA1-Tastin

Plasmid

#11871

Depositor

Michiko Fukuda

Article

Fukuda et al Genes Dev. 1995 May 15. 9(10):1199-210.

Insert

Tastin (TROAP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
Hs.HRAS Q61K

Plasmid

#83187

Purpose

Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61K mutation

Depositor

Dominic Esposito

Insert

HRAS (HRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Q61K

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-1 Sesn3

Plasmid

#61875

Purpose

bacterial expression of a GST-Sesn3 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn3 (Sesn3 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

none

Promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only
SF-1 shRNA #2

Plasmid

#28187

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pCTcon2-dHUH

Plasmid

#235187

Purpose

To display dHUH on yeast surface

Depositor

Alice Ting

Article

Huang et al Proc Natl Acad Sci U S A. 2025 Mar 4;122

Insert

dHUH

Use

Tags

HA, aga2p, and myc

Expression

Yeast

Mutation

Promoter

Available since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 L113A+

Plasmid

#231873

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 L113A+

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 L113A+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L113A, E114Y, N126H, D127E, S136A

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 F108A+

Plasmid

#231875

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F108A+

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 F108A+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108A, T121L, A141P

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSpyC

Plasmid

#220187

Purpose

sgRNA expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBG42

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCytERM-mRhubarb713-N1

Plasmid

#197187

Purpose

Expresses the fusion protein of CytERM-mRhubarb713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-mRhubarb713

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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A0A0M2H872

Plasmid

#163187

Purpose

Inducible expression of UniProt identifier A0A0M2H872

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0M2H872

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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