Skip to main content
Addgene
Showing: 1081 - 1100 of 2232 results
  1. GLuc-PM

    Plasmid
    #164783
    Purpose
    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Insert
    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
    Use
    Tags
    GLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. NanoLuc-PM

    Plasmid
    #164784
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
    Use
    Tags
    NanoLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. NanoLuc-PM-Venus

    Plasmid
    #164786
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
    Depositor
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
    Use
    Tags
    NanoLuc and mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. GLuc-PM-Venus

    Plasmid
    #164785
    Purpose
    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
    Depositor
    Insert
    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Gaussia princeps, Human)
    Use
    Tags
    GLuc and mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3-β2AR-μDpTT

    Plasmid
    #182326
    Purpose
    Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Insert
    beta2AR-μDronpa-TagRFP-T (ADRB2 Human)
    Use
    Tags
    Full-length human beta2 adrenergic receptor
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pBF_HsPepT1

    Plasmid
    #73943
    Purpose
    Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag
    Depositor
    Insert
    PepT1 (SLC15A1 Human)
    Use
    Make rna for xenopus oocyte injection
    Tags
    Flag
    Expression
    Mutation
    Promoter
    beta globin
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pBF_HsPepT2

    Plasmid
    #73944
    Purpose
    Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag
    Depositor
    Insert
    PepT2 (SLC15A2 Human)
    Use
    Make rna for xenopus oocyte injection
    Tags
    Flag
    Expression
    Mutation
    Promoter
    beta globin
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. ADRB2-DuET

    Plasmid
    #213181
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 1081 - 1100 of 2232 results