We narrowed to 1,799 results for: pDest

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actc1b-mKate2-CDC42-DN

Plasmid

#109532

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2CG NBT:NRGIIIa:polyA

Plasmid

#193012

Purpose

Tol2 transgenic construct that will express human Neuregulin1 Type IIIa in neurons

Depositor

William Talbot

Article

Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

Inserts

NBT
NRG1 type IIIa (NRG1 Human)

Use

Transposon transgenesis

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-CDC42-DN

Plasmid

#109584

Purpose

Multisite gateway vector for 3' tagging with dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-SERCA1

Plasmid

#109498

Purpose

Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

SERCA1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EEA1

Plasmid

#109508

Purpose

Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EEA1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-ARF1-DN

Plasmid

#109536

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ARF1 (arf1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-ARF6-DN

Plasmid

#109538

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF6

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ARF6 (arf6a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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2xmyc-RAB10 (T73A)

Plasmid

#244984

Purpose

Express RAB10 in mammalian cells with 2xmyc tag and T73A mutation

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

RAB10 (RAB10 Human)

Tags

2xmyc

Expression

Mammalian

Mutation

T73A

Promoter

CMV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pEXP(FLAG/HA-NSP1-K164-H165A)

Plasmid

#188782

Purpose

Expresses FLAG/HA-NSP1-K164-H165A in mammalian cells (SARS-CoV-2 mutant NSP1)

Depositor

Markus Hafner

Article

Anastasakis et al PLoS One. 2024 Jan 26;19(1):e0297262. do

Insert

FLAG/HA-NSP1-K164-H165A (SARS-CoV-2)

Use

Frt site to insert into hek293 t-rex genome

Tags

FLAG/HA

Expression

Mammalian

Mutation

Changed Lysine 164 to Alanine and Histidine 165 t…

Promoter

CMV Dox inducible

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2CG NBT:NRGIIIa_R551Q:polyA

Plasmid

#193013

Purpose

Tol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neurons

Depositor

William Talbot

Article

Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

Inserts

NBT
NRG1 type IIIa R551Q (NRG1 Human)

Use

Transposon transgenesis

Expression

Mammalian

Mutation

ENST00000287842, NM_013956, c.1652G>A, p.(Arg5…

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGAPDH::sNluc1

Plasmid

#187221

Purpose

A plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Overexpression

Promoter

GAPDH

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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ATX3-JD

Plasmid

#184247

Purpose

Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)

Depositor

Sandra Macedo Ribeiro

Article

Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

Insert

Ataxin-3 Josephin domain (ATXN3 Human)

Tags

6x His-Tag and TEV cleavage site

Expression

Bacterial

Mutation

Premature stop codon V183X

Promoter

T7 promoter

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ATX3-D1

Plasmid

#184246

Purpose

Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)

Depositor

Sandra Macedo Ribeiro

Article

Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

Insert

Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)

Tags

6x His-Tag and TEV cleavage site

Expression

Bacterial

Mutation

Premature stop codon N263X

Promoter

T7 promoter

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-jph1a

Plasmid

#109497

Purpose

Muscle specific zebrafish expression of mKate2 fused to junctophilin 1a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

jph1a (jph1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-SERCA1

Plasmid

#109552

Purpose

Multisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

sarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X65-527: His6-tev-Hs.CALM1(2-148)

Plasmid

#159693

Purpose

E. coli protein expression of His6-tev-Hs.CALM1(2-148)

Depositor

Dominic Esposito

Article

Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

Insert

His6-tev-Hs.CALM1(2-148) (CALM1 Human)

Tags

His6-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218857

Purpose

Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Tags

QF fusion protein

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166013

Purpose

mammalian expression of MERS-CoV soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

MERS-CoV S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166015

Purpose

mammalian expression of HCoV-OC43 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-OC43 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166017

Purpose

mammalian expression of HCoV-NL63 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-NL63 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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