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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#7

Plasmid

#173991

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #7 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #7

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-platypus_min2

Plasmid

#173967

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and platypus minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_3XCoordinatorMutant#5-6-7

Plasmid

#173993

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #5, #6 and #7 mutated

Use

Luciferase

Mutation

3X Coordinator motif mutation #5-6-7

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_3XCoordinatorMutant

Plasmid

#173964

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 3 mutated Coordinator motifs

Use

Luciferase

Mutation

3 mutated Coordinator motifs

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-mouse_min2

Plasmid

#173965

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and mouse minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-lizard_min2

Plasmid

#173969

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and lizard minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-puro

Plasmid

#156169

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-WT-puro

Plasmid

#156170

Purpose

Lentiviral vector for expression of RAP2A-WT, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-V12-puro

Plasmid

#156171

Purpose

Lentiviral vector for expression of RAP2A-V12, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-N17-puro

Plasmid

#156172

Purpose

Lentiviral vector for expression of RAP2A-N17, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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miR3 HsATP10B

Plasmid

#171824

Purpose

transfer plasmid for lentiviral vector production with miR for Hs ATP10B

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Promoter

SFFV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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miR7 MmATP10B

Plasmid

#171825

Purpose

transfer plasmid for lentiviral vector production with miR for Mm ATP10B

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (Atp10b Mouse)

Use

Lentiviral

Promoter

SFFV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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miR5 MmATP10B

Plasmid

#171791

Purpose

transfer plasmid for lentiviral vector production with miR for Mm ATP10B

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (Atp10b Mouse)

Use

Lentiviral

Promoter

SFFV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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miR2 HsATP10B

Plasmid

#171790

Purpose

transfer plasmid for lentiviral vector production with miR for Hs ATP10B

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Promoter

SFFV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXI3

Plasmid

#101518

Purpose

Donor Vector containing FOXI3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXI3 (FOXI3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ASCL3

Plasmid

#101515

Purpose

Donor Vector containing ASCL3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ASCL3 (ASCL3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-HOXB3

Plasmid

#101478

Purpose

Donor Vector containing HOXB3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXB3 (HOXB3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXE3

Plasmid

#101429

Purpose

Donor Vector containing FOXE3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXE3 (FOXE3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-FOXH1

Plasmid

#101431

Purpose

Donor Vector containing FOXH1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXH1 (FOXH1 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-BSX

Plasmid

#101410

Purpose

Donor Vector containing BSX transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

BSX (BSX Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge